Institute of Crop Science and Resource Conservation (INRES), Plant Diseases and Plant Protection, University of Bonn, Nussallee 9, 53115 Bonn, Germany.
Molecular Phytopathology and Mycotoxin Research, University of Goettingen, Grisebachstraße 6, 37077 Goettingen, Germany.
Toxins (Basel). 2019 Sep 21;11(10):556. doi: 10.3390/toxins11100556.
head blight (FHB) epidemics in wheat and contamination with mycotoxins has become an increasing problem over the last decades. This prompted the need for non-invasive and non-destructive techniques to screen cereal grains for infection, which is usually accompanied by mycotoxin contamination. This study tested the potential of hyperspectral imaging to monitor the infection of wheat kernels and flour with three species. Kernels of two wheat varieties inoculated at anthesis with , and were investigated. Hyperspectral images of kernels and flour were taken in the visible-near infrared (VIS-NIR) (400-1000 nm) and short-wave infrared (SWIR) (1000-2500 nm) ranges. The fungal DNA and mycotoxin contents were quantified. Spectral reflectance of -damaged kernels (FDK) was significantly higher than non-inoculated ones. In contrast, spectral reflectance of flour from non-inoculated kernels was higher than that of FDK in the VIS and lower in the NIR and SWIR ranges. Spectral reflectance of kernels was positively correlated with fungal DNA and deoxynivalenol (DON) contents. In the case of the flour, this correlation exceeded = -0.80 in the VIS range. Remarkable peaks of correlation appeared at 1193, 1231, 1446 to 1465, and 1742 to 2500 nm in the SWIR range.
过去几十年来，小麦的头部枯萎病（FHB）流行和真菌毒素污染已成为一个日益严重的问题。这促使人们需要采用非侵入性和非破坏性的技术来筛选谷物是否受到感染，因为这种感染通常伴随着真菌毒素污染。本研究测试了高光谱成像技术在监测三种真菌对小麦籽粒和面粉感染的潜力。研究了两个小麦品种的籽粒，这些品种在开花期被接种了 和 。采集了籽粒和面粉的可见-近红外（VIS-NIR）（400-1000nm）和短波红外（SWIR）（1000-2500nm）范围内的高光谱图像。定量了真菌 DNA 和真菌毒素含量。与未接种的籽粒相比， -损伤的籽粒（FDK）的光谱反射率显著更高。相比之下，未接种的籽粒的面粉的光谱反射率在 VIS 范围内高于 FDK，而在 NIR 和 SWIR 范围内则较低。籽粒的光谱反射率与真菌 DNA 和脱氧雪腐镰刀菌烯醇（DON）含量呈正相关。对于面粉，这种相关性在 VIS 范围内超过了 = -0.80。在 SWIR 范围内，出现了一些显著的相关峰值，其波长分别为 1193nm、1231nm、1446nm 至 1465nm 以及 1742nm 至 2500nm。
