• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

聚乙烯生物降解菌株LBX-2的全基因组序列及特征分析

Complete Genome Sequence and Characterization of a Polyethylene Biodegradation Strain, LBX-2.

作者信息

Shao Huanhuan, Chen Meiju, Fei Xueting, Zhang Ronglin, Zhong Yue, Ni Weimin, Tao Xiang, He XinYi, Zhang Erliang, Yong Bin, Tan Xuemei

机构信息

College of Life Sciences, Sichuan Normal University, Chengdu 610101, China.

College of Life Sciences, Sichuan University, Chengdu 610064, China.

出版信息

Microorganisms. 2019 Sep 22;7(10):379. doi: 10.3390/microorganisms7100379.

DOI:10.3390/microorganisms7100379
PMID:31546741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6843780/
Abstract

A bacterial strain, LBX-2, was isolated from a soil sample in Chengdu, China. LBX-2 is an aerobic and Gram-positive microorganism that is capable of using the polyethylene as the sole carbon source. Results of scanning electron microscopy and tensile tests indicated that LBX-2 could cause the damages to polyethylene (PE). Suspension culture of LBX-2 resulted in the weight loss in the PE powder over a 15-day period. The bacterial growth curve assay clearly demonstrated the utilization of n-hexadecane and n-octadecane for the strain LBX-2. Phylogenetic analysis showed that it was grouped in the same clade as belonging to . The complete genome of strain LBX-2 consists of a chromosome of 7,210,477 bp and a linear plasmid of 336,677 bp. Compared with other strains of , the genome size of LBX-2 was smaller than the average but its guanine and cytosine content (72.47%) was higher than the others. The Non-Redundant Protein Database (NR), Kyoto Encyclopedia of Genes and Genomes (KEGG), SwissProt, Gene Ontology (GO) and Clusters of Orthologous Groups (COG) annotations provided information on the specific functions of encoded proteins. A total of 21 monooxygenase and 22 dioxygenase genes were found in its genome. Synteny comparison with the genome of A3(2) revealed a low overall genetic diversity between them. This study provides valuable information to reveal the underlying mechanisms on PE degradation by LBX-2.

摘要

从中国成都的一份土壤样本中分离出了一株细菌菌株LBX-2。LBX-2是一种需氧革兰氏阳性微生物，能够以聚乙烯作为唯一碳源。扫描电子显微镜和拉伸试验结果表明，LBX-2会对聚乙烯（PE）造成损害。LBX-2的悬浮培养导致PE粉末在15天内重量减轻。细菌生长曲线分析清楚地证明了LBX-2菌株对正十六烷和正十八烷的利用。系统发育分析表明，它与属于……的菌株归为同一进化枝。LBX-2菌株的完整基因组由一条7,210,477 bp的染色体和一条336,677 bp的线性质粒组成。与其他……菌株相比，LBX-2的基因组大小小于平均水平，但其鸟嘌呤和胞嘧啶含量（72.47%）高于其他菌株。非冗余蛋白质数据库（NR）、京都基因与基因组百科全书（KEGG）、瑞士蛋白质数据库（SwissProt）、基因本体论（GO）和直系同源基因簇（COG）注释提供了有关编码蛋白质特定功能的信息。在其基因组中总共发现了21个单加氧酶基因和22个双加氧酶基因。与A3(2)的基因组进行共线性比较，发现它们之间的总体遗传多样性较低。本研究为揭示LBX-2降解PE的潜在机制提供了有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/f41a9fe08428/microorganisms-07-00379-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/71bc8319d68e/microorganisms-07-00379-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/edfb46a46259/microorganisms-07-00379-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/7ae479d2c7a4/microorganisms-07-00379-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/0b2e94ae445d/microorganisms-07-00379-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/d90b6595e6bc/microorganisms-07-00379-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/f41a9fe08428/microorganisms-07-00379-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/71bc8319d68e/microorganisms-07-00379-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/edfb46a46259/microorganisms-07-00379-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/7ae479d2c7a4/microorganisms-07-00379-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/0b2e94ae445d/microorganisms-07-00379-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/d90b6595e6bc/microorganisms-07-00379-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8bf/6843780/f41a9fe08428/microorganisms-07-00379-g008.jpg

相似文献

1
Complete Genome Sequence and Characterization of a Polyethylene Biodegradation Strain, LBX-2.聚乙烯生物降解菌株LBX-2的全基因组序列及特征分析
Microorganisms. 2019 Sep 22;7(10):379. doi: 10.3390/microorganisms7100379.
2
Genomic Exploration of a Chitinolytic PMB5 Strain from European mantis ().来自欧洲螳螂的几丁质分解性PMB5菌株的基因组探索
Curr Issues Mol Biol. 2024 Aug 24;46(9):9359-9375. doi: 10.3390/cimb46090554.
3
Bioremediation of Carbendazim, a Benzimidazole Fungicide Using Brevibacillus borstelensis and Streptomyces albogriseolus Together.使用博斯腾短芽孢杆菌和浅灰链霉菌共同对苯并咪唑类杀菌剂多菌灵进行生物修复
Curr Pharm Biotechnol. 2015;17(2):185-9. doi: 10.2174/1389201016666150930115737.
4
Degradation of latex and of natural rubber by Streptomyces strain La 7.链霉菌菌株La 7对乳胶和天然橡胶的降解作用
Syst Appl Microbiol. 2000 Oct;23(3):433-41. doi: 10.1016/S0723-2020(00)80075-2.
5
A novel member of the subtilisin-like protease family from Streptomyces albogriseolus.来自浅灰链霉菌的枯草杆菌蛋白酶样蛋白酶家族的一个新成员。
J Bacteriol. 1997 Jan;179(2):430-8. doi: 10.1128/jb.179.2.430-438.1997.
6
Complete genome sequence of soil actinobacteria Streptomyces cavourensis TJ430.土壤放线菌解硫小链霉菌 TJ430 的全基因组序列。
J Basic Microbiol. 2018 Dec;58(12):1083-1090. doi: 10.1002/jobm.201800181. Epub 2018 Sep 21.
7
Identification and characterization of genes from Streptomyces sp. strain K30 responsible for clear zone formation on natural rubber latex and poly(cis-1,4-isoprene) rubber degradation.来自链霉菌属菌株K30的、负责在天然橡胶胶乳和聚（顺式-1,4-异戊二烯）橡胶降解过程中形成透明圈的基因的鉴定与表征。
Biomacromolecules. 2005 Jan-Feb;6(1):180-8. doi: 10.1021/bm0496110.
8
The location and deletion of the genes which code for SSI-like protease inhibitors in Streptomyces species.链霉菌属中编码类SSI蛋白酶抑制剂的基因的定位与缺失
FEMS Microbiol Lett. 1996 May 15;139(1):37-42. doi: 10.1111/j.1574-6968.1996.tb08176.x.
9
Reclassification of 15 species as synonyms of or .将15个物种重新分类为或的同义词。
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004718. Epub 2021 Mar 1.
10
Brevibacillus borstelensis and Streptomyces albogriseolus have roles to play in degradation of herbicide, sulfosulfuron.博斯德短芽孢杆菌和浅灰链霉菌在除草剂甲磺隆的降解过程中发挥作用。
3 Biotech. 2016 Dec;6(2):246. doi: 10.1007/s13205-016-0562-z. Epub 2016 Nov 16.

引用本文的文献

1
Responses of natural plastisphere community and zooplankton to microplastic pollution: a review on novel remediation strategies.天然塑料球群落和浮游动物对微塑料污染的响应：新型修复策略综述
Arch Microbiol. 2025 May 7;207(6):136. doi: 10.1007/s00203-025-04334-y.
2
Degradation of polyvinyl chloride (PVC) microplastics employing the actinobacterial strain Streptomyces gobitricini.利用放线菌菌株戈壁链霉菌降解聚氯乙烯（PVC）微塑料
Biodegradation. 2025 Feb 7;36(1):19. doi: 10.1007/s10532-025-10115-7.
3
Role of Humic Substances in the (Bio)Degradation of Synthetic Polymers under Environmental Conditions.

本文引用的文献

1
Dos and Do Nots When Assessing the Biodegradation of Plastics.评估塑料生物降解时的注意事项
Environ Sci Technol. 2019 Sep 3;53(17):9967-9969. doi: 10.1021/acs.est.9b04513. Epub 2019 Aug 16.
2
The complete genome sequence of Ethanoligenens harbinense reveals the metabolic pathway of acetate-ethanol fermentation: A novel understanding of the principles of anaerobic biotechnology.乙醇杆菌全基因组序列揭示了乙酸-乙醇发酵的代谢途径：对厌氧生物技术原理的新认识。
Environ Int. 2019 Oct;131:105053. doi: 10.1016/j.envint.2019.105053. Epub 2019 Jul 26.
3
Investigation of biodegradation potentials of high density polyethylene degrading marine bacteria isolated from the coastal regions of Tamil Nadu, India.
腐殖质在环境条件下对合成聚合物（生物）降解中的作用
Microorganisms. 2024 Oct 6;12(10):2024. doi: 10.3390/microorganisms12102024.
4
Genomic Exploration of a Chitinolytic PMB5 Strain from European mantis ().来自欧洲螳螂的几丁质分解性PMB5菌株的基因组探索
Curr Issues Mol Biol. 2024 Aug 24;46(9):9359-9375. doi: 10.3390/cimb46090554.
5
Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation.从生物勘探肠道菌株中分离和鉴定 Bacillus safensis CGK192 和 Bacillus australimaris CGK221 对塑料（HDPE）的降解作用。
Biotechnol Lett. 2024 Aug;46(4):671-689. doi: 10.1007/s10529-024-03486-z. Epub 2024 May 6.
6
Microorganism-mediated biodegradation for effective management and/or removal of micro-plastics from the environment: a comprehensive review.微生物介导的生物降解在有效管理和/或去除环境中的微塑料方面的作用：全面综述。
Arch Microbiol. 2024 Apr 1;206(4):198. doi: 10.1007/s00203-024-03904-w.
7
Marine-Derived Actinomycetes: Biodegradation of Plastics and Formation of PHA Bioplastics-A Circular Bioeconomy Approach.海洋来源放线菌：塑料的生物降解和 PHB 生物塑料的形成——一种循环生物经济方法。
Mar Drugs. 2022 Dec 1;20(12):760. doi: 10.3390/md20120760.
8
as Potential Synthetic Polymer Degraders: A Systematic Review.作为潜在的合成聚合物降解剂：一项系统综述。
Bioengineering (Basel). 2021 Oct 23;8(11):154. doi: 10.3390/bioengineering8110154.
9
The Terrestrial Plastisphere: Diversity and Polymer-Colonizing Potential of Plastic-Associated Microbial Communities in Soil.陆地塑料圈：土壤中与塑料相关的微生物群落的多样性及聚合物定殖潜力
Microorganisms. 2021 Sep 3;9(9):1876. doi: 10.3390/microorganisms9091876.
10
Comparative Genome Analysis Provides Molecular Evidence for Reclassification of the Photosynthetic Bacterium EBL0706 as a Strain of .比较基因组分析为光合细菌EBL0706重新分类为[具体菌种]的菌株提供了分子证据。
Microorganisms. 2021 Aug 17;9(8):1754. doi: 10.3390/microorganisms9081754.
印度泰米尔纳德邦沿海地区分离的具有高密度聚乙烯生物降解潜力的海洋细菌的研究。
Mar Pollut Bull. 2019 Jan;138:549-560. doi: 10.1016/j.marpolbul.2018.12.001. Epub 2018 Dec 14.
4
Microplastic in riverine fish is connected to species traits.河鱼体内的微塑料与物种特征有关。
Sci Rep. 2018 Aug 3;8(1):11639. doi: 10.1038/s41598-018-29980-9.
5
Actinomycetes benefaction role in soil and plant health.放线菌在土壤和植物健康中的有益作用。
Microb Pathog. 2017 Oct;111:458-467. doi: 10.1016/j.micpath.2017.09.036. Epub 2017 Sep 18.
6
Microplastic transport in soil by earthworms.土壤中蚯蚓对微塑料的传输作用。
Sci Rep. 2017 May 2;7(1):1362. doi: 10.1038/s41598-017-01594-7.
7
Effects of micro- and nanoplastics on aquatic ecosystems: Current research trends and perspectives.微塑料和纳米塑料对水生生态系统的影响：当前的研究趋势和展望。
Mar Pollut Bull. 2017 Nov 30;124(2):624-632. doi: 10.1016/j.marpolbul.2017.01.070. Epub 2017 Feb 20.
8
Biodegradation of polyethylene microplastics by the marine fungus Zalerion maritimum.海洋真菌 Zalerion maritimum 对聚乙烯微塑料的生物降解作用。
Sci Total Environ. 2017 May 15;586:10-15. doi: 10.1016/j.scitotenv.2017.02.017. Epub 2017 Feb 12.
9
Quantitative investigation of the mechanisms of microplastics and nanoplastics toward zebrafish larvae locomotor activity.定量研究微塑料和纳米塑料对斑马鱼幼鱼游动活动的作用机制。
Sci Total Environ. 2017 Apr 15;584-585:1022-1031. doi: 10.1016/j.scitotenv.2017.01.156. Epub 2017 Feb 6.
10
TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2).TrpM，一种调控天蓝色链霉菌A3(2)中色氨酸生物合成及形态生理分化的小蛋白。
PLoS One. 2016 Sep 26;11(9):e0163422. doi: 10.1371/journal.pone.0163422. eCollection 2016.