Antipyretic, Antinociceptive, and Anti-Inflammatory Activities from Leaf Extract in Experimental Wister Rats.

leaves have traditionally been utilized for relieving body aches, headaches and fever. Based on its uses, the present study was designed to investigate the antinociceptive, antipyretic and anti-edematogenic activities from leaves' methanol extract (PBME) in Wister rats. The thermal (hot plate) and chemical (acetic acid-induced writhing and formalin test) models for antinociceptive effects, and the Brewer's yeast induced hyperthermia test for antipyretic action and rat paw edema by carrageenan for anti-edematogenic activity, were applied for PBME at different dose levels. The acute toxicity of PBME through the oral route was performed to determine the lethal dose. PBME significantly and dose-dependently reduced pyrexia and diminished edema volume, which depicted its antipyretic and anti-edematogenic effects respectively. The inhibition of writhing reflex, increased reaction latency and reduced frequency of licking indicated that PBME has significant dose-dependent antinociceptive activity. methanol extract at 4000 mg/kg shows no sign of toxicity, which is a considerable, good margin of safety. The study illustrated the antipyretic, antinociceptive and anti-inflammatory potential of leaf extract with a safety margin, and validated its traditional use to alleviate fever, pain, and inflammation.