Aryal Sushant, Adhikari Balkrishna, Panthi Kasmira, Aryal Pramod, Mallik Shyam Kumar, Bhusal Ram Prasad, Salehi Bahare, Setzer William N, Sharifi-Rad Javad, Koirala Niranjan
Department of Pharmacy, Universal College of Medical Sciences, Tribhuvan University, Bhairahawa, Rupandehi 32900, Nepal.
Department of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu 44600, Nepal.
Medicines (Basel). 2019 Sep 20;6(4):96. doi: 10.3390/medicines6040096.
leaves have traditionally been utilized for relieving body aches, headaches and fever. Based on its uses, the present study was designed to investigate the antinociceptive, antipyretic and anti-edematogenic activities from leaves' methanol extract (PBME) in Wister rats. The thermal (hot plate) and chemical (acetic acid-induced writhing and formalin test) models for antinociceptive effects, and the Brewer's yeast induced hyperthermia test for antipyretic action and rat paw edema by carrageenan for anti-edematogenic activity, were applied for PBME at different dose levels. The acute toxicity of PBME through the oral route was performed to determine the lethal dose. PBME significantly and dose-dependently reduced pyrexia and diminished edema volume, which depicted its antipyretic and anti-edematogenic effects respectively. The inhibition of writhing reflex, increased reaction latency and reduced frequency of licking indicated that PBME has significant dose-dependent antinociceptive activity. methanol extract at 4000 mg/kg shows no sign of toxicity, which is a considerable, good margin of safety. The study illustrated the antipyretic, antinociceptive and anti-inflammatory potential of leaf extract with a safety margin, and validated its traditional use to alleviate fever, pain, and inflammation.
传统上，树叶被用于缓解身体疼痛、头痛和发烧。基于其用途，本研究旨在调查紫藤叶甲醇提取物（PBME）对Wistar大鼠的抗伤害感受、解热和抗水肿活性。采用热（热板）和化学（乙酸诱导扭体和福尔马林试验）模型评估抗伤害感受作用，采用啤酒酵母诱导的体温过高试验评估解热作用，采用角叉菜胶诱导大鼠足肿胀试验评估抗水肿活性，对不同剂量水平的PBME进行检测。通过口服途径进行PBME的急性毒性试验以确定致死剂量。PBME显著且剂量依赖性地降低了发热并减小了水肿体积，分别显示出其解热和抗水肿作用。扭体反射的抑制、反应潜伏期的延长和舔舐频率的降低表明PBME具有显著的剂量依赖性抗伤害感受活性。4000mg/kg的甲醇提取物未显示出毒性迹象，这是一个相当可观的良好安全范围。该研究阐明了树叶提取物具有解热、抗伤害感受和抗炎潜力以及安全范围，并验证了其用于缓解发热、疼痛和炎症的传统用途。
