Duba Adrian, Goriewa-Duba Klaudia, Wachowska Urszula, Głowacka Katarzyna, Wiwart Marian
Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-719 Olsztyn, Poland.
Department of Plant Breeding and Seed Production, University of Warmia and Mazury in Olsztyn, pl. Łódzki 3, 10-724 Olsztyn, Poland.
Plants (Basel). 2019 Sep 23;8(10):360. doi: 10.3390/plants8100360.
In wheat, resistance to is conditioned by anatomical, morphological, and physiological traits. The aim of this study was to evaluate selected elements of constitutive barriers in common wheat, spelt, Polish wheat, emmer, and einkorn. The activity of the phenylalanine ammonia-lyase (PAL) enzyme and rate of reactive oxygen species (ROS) production were evaluated in the tissues of common wheat and spelt inoculated with . Most of the relict wheat species were more abundant in morphological barriers than common wheat. penetrated constitutive barriers, which increased PAL activity and intensified ROS production 24 h after inoculation in wheat tissues. The lowest increase in PAL activity after inoculation was observed in cv. Sumai3, which resistance is based on limiting the spread of within the spike. Spelt line 581 glumes were characterized by the highest concentration of ROS 24 h after inoculation. The ROS content remained high for five days. The results of this study indicate that high trichome density plays a key role in resistance to pathogens. In the resistant spelt line with effective constitutive barriers, PAL activity and ROS content were higher than those observed in susceptible wheats after inoculation with .
在小麦中，对[病原体名称未给出]的抗性由解剖学、形态学和生理学特征决定。本研究的目的是评估普通小麦、斯佩尔特小麦、波兰小麦、二粒小麦和一粒小麦中组成型屏障的选定要素。在接种了[病原体名称未给出]的普通小麦和斯佩尔特小麦组织中评估了苯丙氨酸解氨酶（PAL）的活性和活性氧（ROS）的产生速率。大多数残留小麦品种在形态屏障方面比普通小麦更为丰富。[病原体名称未给出]穿透了组成型屏障，这在接种后24小时增加了小麦组织中的PAL活性并加剧了ROS的产生。接种后PAL活性增加最低的是苏麦3号品种，其抗性基于限制[病原体名称未给出]在穗内的传播。斯佩尔特小麦品系581的颖片在接种后24小时具有最高的ROS浓度。ROS含量在五天内一直保持较高水平。本研究结果表明，高表皮毛密度在对病原体的抗性中起关键作用。在具有有效组成型屏障的抗性斯佩尔特小麦品系中，接种[病原体名称未给出]后，PAL活性和ROS含量高于易感小麦。
