Department of Biochemistry, Punjab Agricultural University, Ludhiana, Punjab, India.
Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, India.
PeerJ. 2023 Mar 28;11:e15134. doi: 10.7717/peerj.15134. eCollection 2023.
Biotic stress due to fungal infection is detrimental to the growth and development of chickpea. In our study, two chickpea genotypes (resistant) and PBG5 (susceptible) were inoculated with (1 × 10 spore mL) of nectrotrophic fungus at seedling stage. These seedlings were evaluated for morphological, ultrastructural, and molecular differences after 3, 5 and 7 days post inoculation (dpi). Visual symptoms were recorded in terms of water-soaked lesions, rotten pods and twigs with fungal colonies. Light and scanning electron microscopy (SEM) revealed the differences in number of stomata, hyphal network and extent of topographical damage in resistant () and susceptible (PBG5) genotypes, which were validated by stomatal index studies done by using fluorescence microscopy in the infection process of in leaves of both chickpea genotypes. In case of control (water inoculated) samples, there were differences in PCR analysis done using five primers for screening the genetic variations between two genotypes. The presence of a Botrytis responsive gene (LrWRKY) of size ~300 bp was observed in uninoculated resistant genotype which might have a role in resistance against Botrytis grey mould. The present investigation provides information about the variation in the infection process of in two genotypes which can be further exploited to develop robust and effective strategies to manage grey mould disease.
真菌侵染导致的生物胁迫会对鹰嘴豆的生长和发育造成损害。在我们的研究中，将两种鹰嘴豆基因型（抗性）和 PBG5（敏感性）用（1×10 个孢子 mL）的坏死型真菌在幼苗期接种。在接种后 3、5 和 7 天，对这些幼苗进行形态学、超微结构和分子差异评估。以水渍状病斑、腐烂的豆荚和有真菌菌落的嫩枝为特征，记录可见症状。通过光镜和扫描电子显微镜（SEM）观察到，在抗性（）和敏感性（PBG5）基因型中，气孔数量、菌丝网络和地形损伤程度存在差异，这通过荧光显微镜观察感染过程中气孔指数研究得到了验证。在对照（水接种）样本中，使用 5 个引物进行 PCR 分析，筛选两种基因型之间的遗传变异。在未接种的抗性基因型中观察到大小约为 300bp 的 Botrytis 响应基因（LrWRKY）的存在，该基因可能在抵抗 Botrytis 灰霉病方面发挥作用。本研究提供了两种基因型中 感染过程变化的信息，可以进一步开发稳健有效的策略来管理灰霉病。
