大黄（Rheum ribes L.）提取物抗包虫蚴原头节的体外与离体抗寄生虫作用。
In vitro and Ex vivo Antiparasitic Effect of Rheum ribes L. Extract Against the Hydatid Cyst Protoscoleces.
机构信息
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Social Determinants of Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
出版信息
Infect Disord Drug Targets. 2021;21(6):e170721187993. doi: 10.2174/1871526520666201116094851.
BACKGROUND
Cystic echinococcosis is a zoonotic infection in humans and herbivorous animals occurring worldwide, which is caused by the larva stage of Echinococcus granulosus. Rhubarb (Rheum ribes L.) as an herbal medicine has various therapeutic properties such as antioxidant, anticancer, and antimicrobial. With respect to the potential of the biological activities of this plant in traditional and modern medicine, we aim to examine its protoscolicidal effects against E. granulosus protoscoleces in vitro and ex vivo.
METHODS
Collected protoscoleces from liver hydatid cysts of infected sheep were exposed to the different concentrations of the extract (225, 450, 900 mg/mL) for 5-60 min in vitro and ex vivo. Then, by using the eosin exclusion assay, the viability of protoscoleces was studied.
RESULTS
R. ribes extract had a potent protoscolicidal activity in vitro ; at 450 and 900 mg/ml, it killed 56.3 and 100% of protoscoleces, respectively, after 10 min of exposure. In ex vivo assay, the extract needed more time to kill the protoscoleces than the in vitro; so that at the concentration of 900 mg/mL, all protoscoleces were killed after 15 minutes.
CONCLUSION
The obtained results exhibited the potent protoscolicidal effects of R. ribes extract, particularly at the concentration of 900 mg/ml, which completely killed the parasite after <15 min of exposure. However, more supplementary studies are required to verify these findings by assessing animal models and clinical subjects.
背景
囊型包虫病是一种人兽共患的感染病，在全球范围内发生，由细粒棘球绦虫的幼虫阶段引起。大黄（Rheum ribes L.）作为一种草药，具有抗氧化、抗癌和抗菌等多种治疗特性。鉴于该植物在传统和现代医学中的生物活性的潜力，我们旨在研究其对体外和体内细粒棘球蚴原头蚴的杀原头蚴作用。
方法
从感染绵羊的肝包虫囊肿中收集原头蚴，将其暴露于不同浓度的提取物（225、450、900mg/ml）中 5-60 分钟，进行体外和体内实验。然后，通过伊红排斥试验研究原头蚴的活力。
结果
大黄提取物在体外具有很强的杀原头蚴活性；在 450 和 900mg/ml 浓度下，暴露 10 分钟后分别杀死 56.3%和 100%的原头蚴。在体内实验中，提取物杀死原头蚴所需的时间比体外实验长；因此，在 900mg/ml 浓度下，所有原头蚴在 15 分钟后被杀死。
结论
所得结果表明大黄提取物具有很强的杀原头蚴作用，特别是在 900mg/ml 浓度下，暴露<15 分钟即可完全杀死寄生虫。然而，需要更多的补充研究来通过评估动物模型和临床对象来验证这些发现。
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