碳点的生物发光共振能量转移自荧光。

Self-illumination of Carbon Dots by Bioluminescence Resonance Energy Transfer.

机构信息

School of Biomedical Engineering, University of Western Ontario, London, Ontario, N6A 5B9, Canada.

Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario, N6A 5B9, Canada.

出版信息

Sci Rep. 2019 Sep 24;9(1):13796. doi: 10.1038/s41598-019-50242-9.

DOI:10.1038/s41598-019-50242-9

PMID:31551471

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6760201/

Abstract

Carbon-dots (CDs), the emerging fluorescent nanoparticles, show special multicolor properties, chemical stability, and biocompatibility, and are considered as the new and advanced imaging probe in replacement of molecular fluorophores and semiconductor quantum dots. However, the requirement of external high power light source limits the application of fluorescent nanomaterials in bio-imaging. The present study aims to take advantage of bioluminescence resonance energy transfer mechanism (BRET) in creating self-illuminating C-dots. Renilla luciferase (Rluc) is chosen as the BRET donor molecule. Conjugation of Renilla luciferase and C-dots is necessary to keep their distance close for energy transfer. The optimal condition for achieving BRET is investigated by studying the effects of different factors on the performance of BRET, including the type of conjugation, concentration of carbon dots, and conjugation time. The linear relationship of BRET efficiency as a function of the amount of C-dots in the range of 0.20-0.80 mg/mL is observed. The self-illuminating carbon dots could be applied in bioimaging avoiding the tissue damage from the external high power light source.

摘要

碳点（CDs）作为新兴的荧光纳米粒子，具有特殊的多色性质、化学稳定性和生物相容性，被认为是替代分子荧光团和半导体量子点的新型先进成像探针。然而，对外部高功率光源的需求限制了荧光纳米材料在生物成像中的应用。本研究旨在利用生物发光共振能量转移机制（BRET）来创建自发光碳点。海肾荧光素酶（Rluc）被选为 BRET 供体分子。为了保持能量转移的近距离，需要对 Renilla 荧光素酶和碳点进行连接。通过研究不同因素对 BRET 性能的影响，包括连接类型、碳点浓度和连接时间，来探索实现 BRET 的最佳条件。观察到 BRET 效率与 0.20-0.80mg/mL 范围内碳点量之间呈线性关系。自发光碳点可应用于生物成像，避免了外部高功率光源对组织的损伤。