Liu Jinling, Liu Aimin
Department of Biology, Center for Cellular Dynamics, Eberly College of Science, Huck Institute of Life Sciences, The Penn State University, Pennsylvania, PA, USA.
Methods Mol Biol. 2020;2047:475-489. doi: 10.1007/978-1-4939-9732-9_27.
During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of Immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.
在发育过程中，小鼠大脑逐渐被划分为功能不同的区域。随后，响应各种诱导信号产生了众多神经元和神经胶质细胞类型。每个细胞都表达一组独特的基因组合，这些基因编码从转录因子到神经递质的蛋白质，从而确定其在脑功能中的作用。为了理解这些重要且高度复杂的过程，准确地定位产生这些蛋白质的各种蛋白质和细胞至关重要。在本章中，我们将介绍免疫组织化学技术，该技术可检测特定蛋白质的定位，以及RNA原位杂交技术，该技术能够使特定mRNA可视化。
