Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Sciences, Sichuan University, Chengdu, China.
Plant Signal Behav. 2019;14(12):1670596. doi: 10.1080/15592324.2019.1670596. Epub 2019 Sep 25.
Plants growing in natural habitats have evolved a wide range of mechanisms to copy with environmental challenging, including biotic and abiotic stresses. Abiotic stresses-induced increases in Abscisic acid (ABA) levels in plants suffering from stresses, including drought, cold or heat stress. To explore the function of the core components in ABA signaling, we used the overexpression of transgenic plants to expose in heat or cold stress. In this study, overexpression of or (-OE or -OE) transgenic plants had higher germination and survival rate than the wild-type (WT) Arabidopsis, indicating that they are both positively responsive to the high temperature. And the heat shock genes and were significantly induced by RCAR12 or RCAR13. Further, the results inferred that the over-expression of or could tolerance the cold stress, through induction CBFs expressions, the cold-responsive genes when plants were challenged the cold tress. And when complementation of to the 1124 mutant (R12:1124), the results indicated that RCAR12 could recover the insensitivity of 1124 to heat and cold stresses. Hence, we propose that RCAR12 and RCAR13, the ABA receptors, may play the positive roles in regulating the extreme temperature, including cold and high temperature in Arabidopsis.
在自然栖息地生长的植物已经进化出了广泛的机制来应对环境挑战，包括生物和非生物胁迫。非生物胁迫会导致植物中脱落酸（ABA）水平升高，而植物在遭受干旱、寒冷或热胁迫时会产生 ABA。为了探索 ABA 信号转导核心成分的功能，我们使用过表达转基因植物来暴露在高温或低温胁迫下。在这项研究中，过表达 或 （-OE 或 -OE）的转基因植物比野生型（WT）拟南芥具有更高的萌发率和存活率，这表明它们都对高温有正向响应。并且热休克基因 和 被 RCAR12 或 RCAR13 显著诱导。进一步的结果推断，过表达 或 可以通过诱导 CBFs 的表达来耐受低温胁迫，当植物受到冷胁迫时，这些基因会被诱导表达。当将 1124 突变体（R12:1124）中的 互补时，结果表明 RCAR12 可以恢复 1124 对热和冷胁迫的不敏感性。因此，我们提出 ABA 受体 RCAR12 和 RCAR13 可能在调节拟南芥的极端温度（包括寒冷和高温）方面发挥积极作用。
