Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, China.
Chinese Academy of Science, Institute of Subtropical Agriculture, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agroecological Processes in Subtropical Region, Scientific Observation and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.
J Anim Physiol Anim Nutr (Berl). 2020 Jan;104(1):269-279. doi: 10.1111/jpn.13212. Epub 2019 Sep 25.
This study was conducted to determine the effects of oral administration with glutamate on metabolism of suckling piglets based on H-Nuclear magnetic resonance ( H NMR) spectroscopy through the level of metabolism. Forty-eight healthy [(Yorkshire × Landrace) × Duroc] piglets born on the same day with a similar birth bodyweight (1.55 ± 0.20 kg) were obtained from six sows (8 piglets per sow). The piglets from each sow were randomly assigned into four treatments (2 piglets per treatment). The piglets were given 0.09 g/kg body weight (BW) of sodium chloride (CN group), 0.03 g/kg BW monosodium glutamate (LMG group), 0.25 g/kg BW monosodium glutamate (MMG group) and 0.50 g/kg BW monosodium glutamate (HMG group) twice a day respectively. An H NMR-based metabolomics' study found that the addition of monosodium glutamate (MSG) significantly reduced serum citrate content in 7-day-old piglets, while HMG significantly increased serum trimethylamine content and significantly reduced unsaturated fat content in 7-day-old piglets (p < .05). The content of glutamine, trimethylamine, albumin, choline and urea nitrogen was significantly increased and the creatinine content decreased significantly in the 21-day-old HMG (p < .05). Analysis of serum hormones revealed that glucagon-like peptide-1 (GLP-1) content in the 21-day-old HMG was highest (p < .05). The cholecystokinin (CCK) content in the HMG of 7-day-old piglets was lower than that in the LMG (p < .05), and the CCK content in the serum of the 21-day-old MMG was highest (p < .05). The serum leptin levels in the 21-day-old HMG were the lowest (p < .05). The serum insulin content in the 7-day-old MMG was highest (p < .05). This study suggests that MSG plays an important role in the metabolism of sugar, fat and protein (amino acids). These results provide a theoretical basis for designing piglet feed formulations.
本研究旨在通过代谢水平，利用基于 H-核磁共振（H NMR）光谱的方法，确定谷氨酸口服对哺乳仔猪代谢的影响。从 6 头母猪（每头母猪 8 头仔猪）获得了 48 头同日出生、出生体重相近（1.55±0.20kg）的健康 [(约克夏×长白)×杜洛克] 仔猪。每头母猪的仔猪随机分为 4 个处理（每个处理 2 头仔猪）。仔猪每天分别给予 0.09g/kg 体重（BW）的氯化钠（CN 组）、0.03g/kg BW 谷氨酸单钠（LMG 组）、0.25g/kg BW 谷氨酸单钠（MMG 组）和 0.50g/kg BW 谷氨酸单钠（HMG 组）。基于 H NMR 的代谢组学研究发现，添加谷氨酸单钠（MSG）显著降低了 7 日龄仔猪的血清柠檬酸含量，而 HMG 则显著增加了血清三甲胺含量，并显著降低了 7 日龄仔猪的不饱和脂肪含量（p<0.05）。HMG 组 21 日龄仔猪的谷氨酰胺、三甲胺、白蛋白、胆碱和尿素氮含量显著增加，肌酐含量显著降低（p<0.05）。血清激素分析显示，HMG 组 21 日龄仔猪的胰高血糖素样肽-1（GLP-1）含量最高（p<0.05）。7 日龄仔猪 HMG 组的胆囊收缩素（CCK）含量低于 LMG 组（p<0.05），21 日龄 MMG 组的 CCK 含量最高（p<0.05）。HMG 组 21 日龄仔猪血清瘦素水平最低（p<0.05）。7 日龄 MMG 组仔猪血清胰岛素含量最高（p<0.05）。本研究表明，MSG 在糖、脂肪和蛋白质（氨基酸）代谢中发挥重要作用。这些结果为设计仔猪饲料配方提供了理论依据。
