Katsuragi N, Takizawa N, Murooka Y
J Bacteriol. 1987 May;169(5):2301-6. doi: 10.1128/jb.169.5.2301-2306.1987.
We determined the entire nucleotide sequence of the Klebsiella aerogenes W70 pullulanase gene (pulA) contained on a 4.2-kilobase-pair fragment of plasmid pPB174. The amino acid composition deduced from an open reading frame of 3,288 base pairs agreed closely with that determined for the intracellular pullalanase. The precursor enzyme consisted of 1,096 amino acid residues and contained a hydrophobic N-terminal signal peptide and the consensus sequence for the bacterial prelipoprotein signal peptide cleavage site.
我们测定了质粒pPB174 4.2千碱基对片段上携带的产气克雷伯菌W70支链淀粉酶基因（pulA）的完整核苷酸序列。从3288个碱基对的开放阅读框推导的氨基酸组成与细胞内支链淀粉酶的氨基酸组成非常一致。前体酶由1096个氨基酸残基组成，含有一个疏水的N端信号肽和细菌前脂蛋白信号肽切割位点的共有序列。
