Tundis Rosa, Ursino Claudia, Bonesi Marco, Loizzo Monica R, Sicari Vincenzo, Pellicanò Teresa, Manfredi Ilaria L, Figoli Alberto, Cassano Alfredo
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, I-87036 Rende (Cosenza), Italy.
Institute on Membrane Technology, Italian National Research Council, ITM-CNR, I-87036 Rende (Cosenza), Italy.
Membranes (Basel). 2019 Sep 24;9(10):127. doi: 10.3390/membranes9100127.
This study aimed at evaluating and comparing the chemical profile as well as the antityrosinase and antioxidant activities of ethanol (EtOH) and methanol (MeOH) extracts of L. (Adoxaceae) flowers and leaves in order to discover new candidates for food additives and cosmetic and pharmaceutical products. For this purpose, a novel lower-melting-point ethylene-chlorotrifluoroethylene (LMP ECTFE) nanofiltration (NF) membrane was employed in order to produce the concentrated fractions of . Floral extracts were richer in phytochemicals in comparison to the leaf extracts. The High-performance liquid chromatography (HPLC) profile revealed rutin, quercetin, protocateuchic acid, 3,5-dicaffeoylquinic acid, and neochlorogenic acid as the most abundant compounds. Ferric reducing antioxidant power (FRAP), 2,2'-diphenil-1-picrylhydrazil (DPPH) radical scavenging, and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) tests were used to investigate the antioxidant properties. NF retentate fractions of floral ethanol extracts exerted the highest tyrosinase inhibitory activity with an IC of 53.9 µg/mL and the highest ABTS radical scavenging activity (IC of 46.4 µg/mL). In conclusion, the present investigation revealed the potential benefits of NF application in extracts processing, suggesting the use of retentate fractions as a promising source for antioxidant and tyrosinase inhibitory compounds which could pave the way for future applications.
本研究旨在评估和比较五福花科植物花和叶的乙醇提取物（EtOH）和甲醇提取物（MeOH）的化学特征、抗酪氨酸酶活性和抗氧化活性，以发现食品添加剂、化妆品和药品的新候选物。为此，采用了一种新型低熔点乙烯 - 氯三氟乙烯（LMP ECTFE）纳滤（NF）膜来制备该植物提取物的浓缩馏分。与叶提取物相比，花提取物中的植物化学物质更丰富。高效液相色谱（HPLC）图谱显示芦丁、槲皮素、原儿茶酸、3,5 - 二咖啡酰奎尼酸和新绿原酸是含量最丰富的化合物。采用铁还原抗氧化能力（FRAP）、2,2'-二苯基 - 1 - 苦基肼（DPPH）自由基清除和2,2'-偶氮二（3 - 乙基苯并噻唑啉 - 6 - 磺酸）（ABTS）试验来研究抗氧化性能。花乙醇提取物的纳滤截留馏分表现出最高的酪氨酸酶抑制活性，IC50为53.9 μg/mL，以及最高的ABTS自由基清除活性（IC50为46.4 μg/mL）。总之，本研究揭示了纳滤在该植物提取物加工中的潜在益处，表明截留馏分可作为抗氧化和酪氨酸酶抑制化合物的有前景来源，这可为未来应用铺平道路。
