Budiman Arif, Praditasari Arni, Rahayu Driyanti, Aulifa Diah Lia
Department of Science and Technology Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia.
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia.
J Pharm Bioallied Sci. 2019 Jul-Sep;11(3):216-222. doi: 10.4103/jpbs.JPBS_57_18.
Ultraviolet (UV) radiation from the sun that is composed of UVA and UVB can cause premature aging when exposed to the skin. Black mulberry fruit ( L.) contains anthocyanins as antioxidants that can be protective against UV exposure. The aim of this research was to produce gel formulation from the extract of black mulberry as an antioxidant and sunscreen.
This research started with the maceration process using 96% ethanol solvent. Later, antioxidant activity of the extract was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and compared with vitamin C. Furthermore, the extracts were formulated into gel with variations of hydroxypropyl methylcellulose, sodium carboxymethyl cellulose, Carbopol 934, and extract concentrations. The products were then physically evaluated, including organoleptic evaluation, homogeneity, pH, viscosity, and dispersion, as well as the hedonic test, irritation test, and antioxidant activity test.
The results showed that black mulberry extract had antioxidant activity with the half maximal inhibitory concentration (IC) value of 146.73ppm and its antioxidant strength was 39.6 times lower than that of vitamin C. The formulation with best physical evaluation results was given by formula 4 (Carbopol 934, 1.5%) with extract concentration 0.61%. This formula showed antioxidant activity with an IC value of 104.659ppm. Furthermore, on the basis of hedonic test and irritation test, this formula was the most popular and was categorized as a safe topical gel.
The gel of black mulberry extract had antioxidant activity and was categorized as a safe topical gel.
太阳发出的由UVA和UVB组成的紫外线照射皮肤时会导致皮肤过早老化。黑桑椹果实含有花青素作为抗氧化剂，可抵御紫外线照射。本研究的目的是用黑桑椹提取物制备凝胶制剂作为抗氧化剂和防晒剂。
本研究首先采用96%乙醇溶剂进行浸渍过程。随后，采用1,1-二苯基-2-苦基肼（DPPH）法测定提取物的抗氧化活性，并与维生素C进行比较。此外，将提取物制成凝胶，改变羟丙基甲基纤维素、羧甲基纤维素钠、卡波姆934和提取物浓度。然后对产品进行物理评估，包括感官评估、均匀性、pH值、粘度和分散性，以及快感测试、刺激性测试和抗氧化活性测试。
结果表明，黑桑椹提取物具有抗氧化活性，半数最大抑制浓度（IC）值为146.73ppm，其抗氧化强度比维生素C低39.6倍。物理评估结果最佳的配方是配方4（卡波姆934，1.5%），提取物浓度为0.61%。该配方的IC值为104.659ppm，具有抗氧化活性。此外，基于快感测试和刺激性测试，该配方最受欢迎，被归类为安全的局部用凝胶。
黑桑椹提取物凝胶具有抗氧化活性，被归类为安全的局部用凝胶。
