Xu Chuan, Liu Di, Mei Hong, Hu Jian, Luo Meng
Department of Thoracic Surgery, Guizhou Provincial People's Hospital, Guiyang, Guizhou Province, China.
J Int Med Res. 2019 Nov;47(11):5650-5659. doi: 10.1177/0300060519869423. Epub 2019 Sep 26.
RAD54 homolog B (RAD54B), a member of the SNF2/SWI2 superfamily, is implicated in homologous recombination, and high RAD54B expression predicts the prognostic outcomes of lung adenocarcinoma. However, its role in lung carcinogenesis was unclear so this was determined in the present study.
We evaluated the gene and protein expression of RAD54B in 15 lung adenocarcinoma tissues and matched adjacent healthy lung tissues by real-time PCR, immunohistochemical staining, and western blotting. A549 lung cancer cells were transduced with lentivirus carrying small hairpin RNA (shRNA) against RAD54B (shRAD54B) or control shRNA (shCtrl), and cell proliferation, viability, apoptosis, and caspase 3/7 activity were evaluated.
RAD54B protein expression was significantly higher in lung adenocarcinoma tissues than in healthy lung tissues. gene expression was high in A549 cells but was efficiently knocked down using shRAD54B with an infection efficiency of 80% and a knockdown ratio of 72.2% compared with shCtrl. Suppressing RAD54B expression in A549 cells significantly reduced cell proliferation and caspase 3/7 activity, and significantly increased the apoptotic rate.
RAD54B exerts an oncogenic role in lung cancer cell proliferation.
RAD54同源物B（RAD54B）是SNF2/SWI2超家族的成员，参与同源重组，RAD54B高表达可预测肺腺癌的预后结果。然而，其在肺癌发生中的作用尚不清楚，因此本研究对此进行了确定。
我们通过实时PCR、免疫组织化学染色和蛋白质印迹法评估了15例肺腺癌组织及配对的相邻健康肺组织中RAD54B的基因和蛋白表达。用携带针对RAD54B的小发夹RNA（shRNA）的慢病毒（shRAD54B）或对照shRNA（shCtrl）转导A549肺癌细胞，并评估细胞增殖、活力、凋亡和半胱天冬酶3/7活性。
肺腺癌组织中RAD54B蛋白表达明显高于健康肺组织。A549细胞中基因表达较高，但与shCtrl相比，使用shRAD54B可有效敲低，感染效率为80%，敲低率为72.2%。抑制A549细胞中RAD54B的表达可显著降低细胞增殖和半胱天冬酶3/7活性，并显著提高凋亡率。
RAD54B在肺癌细胞增殖中发挥致癌作用。
