College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Design, Nankai University, Tianjin 300071, P. R. China.
Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, P. R. China.
Chem Commun (Camb). 2019 Oct 10;55(82):12388-12391. doi: 10.1039/c9cc05633e.
Antibodies are widely used both in clinical practice and in research. However, the development of methods to increase the ratio of antibodies to recognize phosphorylated proteins remains challenging. In this study, we report a novel and useful method for the efficient production of antibodies for phosphorylated proteins. Based on our previously developed vaccine adjuvant Nap-GDFDFDY, we prepared hydrogels by the Ca2+-induced self-assembly of a phosphorylated peptide gelator Nap-GDFDFpDY. The hydrogel could protect phosphorylated antigens from being dephosphorylated by endogenous phosphatase, thus selectively increasing the ratio of the antibodies for phosphorylated proteins. Our study provides a useful strategy for the production of antibodies to recognize proteins with specific posttranslational modifications.
抗体在临床实践和研究中都得到了广泛的应用。然而，开发提高抗体识别磷酸化蛋白比例的方法仍然具有挑战性。在这项研究中，我们报告了一种用于高效生产磷酸化蛋白抗体的新方法。基于我们之前开发的疫苗佐剂 Nap-GDFDFDY，我们通过 Ca2+诱导的磷酸化肽凝胶因子 Nap-GDFDFpDY 的自组装制备了水凝胶。该水凝胶可以保护磷酸化抗原免受内源性磷酸酶的去磷酸化，从而选择性地提高磷酸化蛋白抗体的比例。我们的研究为生产识别具有特定翻译后修饰的蛋白质的抗体提供了一种有用的策略。
