Zhou Zhengquan, Yi Qikun, Xia Tingting, Yin Wencui, Kadi Adnan A, Li Jinbo, Zhang Yan
State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
School of Life Sciences, Nanjing University, Nanjing 210023, China.
Org Biomol Chem. 2017 Mar 8;15(10):2191-2198. doi: 10.1039/c6ob02667b.
Multi-functional supramolecular hydrogels have emerged as smart biomaterials for diverse biomedical applications. Here we report a multi-functional supramolecular hydrogel formed by the conjugate of the bioactive GRGDS peptide with biaryltetrazole that is the substrate of photo-click reaction. The hydrogel was used as a biocompatible matrix to encapsulate live cells for 3D culture. The presence of the RGD epitope in the hydrogelator enhanced the interaction of the nanofiber with integrin over-expressing cells, which resulted in the selective enhancement in the miRNA delivery into the encapsulated U87 cells. The intramolecular photo-click reaction of the biaryltetrazole moiety in the hydrogelator leads to a sensitive photo-response of the hydrogel, which allowed photo-degradation of the hydrogel for release of the encapsulated live cells for further bio-assay of the intracellular species.
多功能超分子水凝胶已成为用于各种生物医学应用的智能生物材料。在此，我们报道了一种由生物活性GRGDS肽与双芳基四唑共轭形成的多功能超分子水凝胶，双芳基四唑是光点击反应的底物。该水凝胶用作生物相容性基质来封装活细胞用于三维培养。水凝胶剂中RGD表位的存在增强了纳米纤维与整合素过表达细胞的相互作用，这导致miRNA向封装的U87细胞中的递送选择性增强。水凝胶剂中双芳基四唑部分的分子内光点击反应导致水凝胶产生灵敏的光响应，这使得水凝胶能够光降解以释放封装的活细胞，用于细胞内物质的进一步生物测定。
