School of Public Health, and Key Laboratory of Environment and Human Health of Hebei Medical University, Shijiazhuang 050017, China.
Department of Nutrition, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China.
J Chromatogr A. 2020 Jan 11;1610:460541. doi: 10.1016/j.chroma.2019.460541. Epub 2019 Sep 14.
In the present research, the application of a polypyrrole coating on FeO magnetic nanoparticles (PPy/FeO MNPs) as a sorbent was confirmed. The synthesized magnetic composites were characterized by TEM, FTIR spectroscopy, and XRD. Four β-lactams (oxacillin (OXA), cloxacillin (CLOX), dicloxacillin (DIC), and flucloxacillin (FLU)) were selected as analytes for the experiment. The extracted β-lactams were determined by micellar electrokinetic capillary chromatography-diode array detector (MEKC-DAD). The crucial parameters influencing the extraction efficiency and separation were studied and optimized. Under the optimal conditions, the limits of detection are 1.0 µg L for OXA, CLOX, FLU, and 0.8 µg L for DIC. The calibration curves are linear in the range of 2.5-200.0 µg L. The proposed method was applied for the determination of the β-lactams in water samples with satisfactory results. The intra-day relative standard deviations and the inter-day relative standard deviations range from 1.09% to 4.58% and from 2.95% to 7.80%, respectively. It can be concluded that this method is sensitive, convenient, and feasible for the determination of the β-lactams in water samples.
在本研究中，确认了将聚吡咯涂层应用于 FeO 磁性纳米粒子（PPy/FeO MNPs）作为吸附剂。通过 TEM、FTIR 光谱和 XRD 对合成的磁性复合材料进行了表征。选择四种β-内酰胺类抗生素（苯唑西林（OXA）、氯唑西林（CLOX）、双氯西林（DIC）和氟氯西林（FLU））作为实验分析物。通过胶束电动毛细管色谱-二极管阵列检测器（MEKC-DAD）测定提取的β-内酰胺类抗生素。研究并优化了影响萃取效率和分离的关键参数。在最佳条件下，OXA、CLOX、FLU 的检测限为 1.0µg·L，DIC 的检测限为 0.8µg·L。校准曲线在 2.5-200.0µg·L 范围内呈线性。该方法已应用于水样中β-内酰胺类抗生素的测定，结果令人满意。日内和日间相对标准偏差分别在 1.09%-4.58%和 2.95%-7.80%范围内。可以得出结论，该方法灵敏、方便、可行，可用于水样中β-内酰胺类抗生素的测定。
