Department of Materials Engineering, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
Materials and Chemical Engineering Faculty, Buein Zahra Technical University, Qazvin, Iran.
J Biomater Sci Polym Ed. 2020 Jan;31(1):93-109. doi: 10.1080/09205063.2019.1675226. Epub 2019 Oct 11.
The aim of this study is to fabricate a highly porous scaffold based on gelatin/sodium alginate/45S5 bioglass with improved mechanical strength. Glycidoxypropyltrimethoxysilane (GPTMS) is used as a cross-linker and for the first time a nonionic bio-surfactant (Tween 80) is introduced to the composition to evaluate whether the essential properties of a suitable scaffold for bone substitution can be reached or not. The composite scaffolds are prepared through freeze-drying of suspension containing various ratios of gelatin/sodium alginate/45S5 bioglass. Characterization of fabricated scaffolds is carried out. SEM micrographs reveal that all samples are highly porous however incorporation of 1% tween 80 results in well-shaped pores, with sizes ranging between 100 and 200 µm which is appropriate for tissue regeneration. Compressive strength of foamed scaffolds in contact with body solution has been enhanced from 0.37 to 1.41 MPa due to addition of tween 80. Foamed scaffold reinforced with tween 80 maintained its structural stability within 7 days immersion in simulated body fluid (SBF) despite the absorption of water 15 times its weight. Moreover, calcium phosphate precipitation is well observed on the surface of scaffolds.
本研究旨在制备一种具有改善的机械强度的基于明胶/海藻酸钠/45S5 生物玻璃的高度多孔支架。使用缩水甘油丙基三甲氧基硅烷 (GPTMS) 作为交联剂，并且首次将非离子型生物表面活性剂（吐温 80）引入到组成中，以评估是否可以达到适合骨替代的合适支架的基本特性。通过包含明胶/海藻酸钠/45S5 生物玻璃的各种比例的悬浮液的冷冻干燥来制备复合支架。对制备的支架进行了表征。SEM 显微照片显示，所有样品均为高度多孔，然而，加入 1％吐温 80 会导致形成形状良好的孔，其尺寸在 100 和 200 μm 之间，适合组织再生。由于添加了吐温 80，与体液接触的泡沫支架的抗压强度已从 0.37 提高到 1.41 MPa。尽管吸收了 15 倍于其重量的水，但添加了吐温 80 的泡沫支架仍能在模拟体液（SBF）中浸泡 7 天内保持其结构稳定性。此外，在支架表面上很好地观察到了磷酸钙沉淀。
