School of Life Science and Technology, Xidian University, Xi'an 710126, China.
Int J Mol Sci. 2019 Sep 27;20(19):4816. doi: 10.3390/ijms20194816.
Auxin response factor (ARF) is a transcription factor that can specifically bind to the promoter of auxin-responsive genes in plants and plays an important regulatory role in plant growth and development. The previous studies have predicted 25 genes in () and indicated that SbARFs play complex roles in salt and drought stresses. In this study, we reclassified and analyzed the structures of ARFs in three plants, including sorghum, rice, and . Phylogenetic analyses categorized 73 ARF into five classes. By studying the characterization of the structures, it was found that SbARFs from the same evolutionary branches showed similar motif patterns. Furthermore, the expression patterns of genes during development and temperature stress were investigated in sorghum. Quantitative transcription-quantitative polymerase chain reaction (qRT-PCR) results suggested that they had different expression patterns in vegetative and reproductive organs at various developmental stages. High and low-temperature treatments and qRT-PCR demonstrated some of them changed dramatically along with the increase of treatment time. Additionally, in situ hybridization results displayed that genes were accumulated in vascular tissues under temperature stress. These findings provide evidence that SbARFs may play important roles in sorghum vegetative development, reproductive development, and auxin response to temperature stress.
生长素响应因子 (ARF) 是一种转录因子，能够特异性地结合到植物生长素响应基因的启动子上，在植物生长和发育中发挥重要的调节作用。先前的研究预测了 () 中的 25 个基因，并表明 SbARFs 在盐和干旱胁迫中发挥着复杂的作用。在这项研究中，我们重新分类并分析了三种植物（高粱、水稻和玉米）中的 ARF 结构。系统发育分析将 73 个 ARF 分为五个类。通过研究结构的特征，发现来自同一进化分支的 SbARFs 表现出相似的基序模式。此外，还研究了高粱中 基因在发育和温度胁迫过程中的表达模式。定量转录-聚合酶链反应 (qRT-PCR) 结果表明，它们在不同发育阶段的营养器官和生殖器官中的表达模式不同。高温和低温处理以及 qRT-PCR 表明，随着处理时间的增加，其中一些基因的表达水平发生了显著变化。此外，原位杂交结果显示，在温度胁迫下， 基因在血管组织中积累。这些发现为 SbARFs 可能在高粱营养生长、生殖生长和生长素对温度胁迫的响应中发挥重要作用提供了证据。
