Erikstrup Lise Tornvig, Dinh Khoa Manh, Andersen Paal Skytt, Skov Robert Leo, Kaspersen Kathrine Agergård, Nielsen Kaspar René, Ellermann-Eriksen Svend, Erikstrup Christian
Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark.
Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark.
Clin Epidemiol. 2019 Sep 19;11:885-900. doi: 10.2147/CLEP.S218637. eCollection 2019.
carriage poses an increased risk of infection. The aim of this study was to investigate the colonization of among healthy individuals and to establish a prospective cohort and biobank for research in the health consequences of colonization.
The Danish Blood Donor Carriage Study (DBDSaCS) was established in 2014. So far, a total of 6082 healthy participants have been included with nasal swabs and repeated swabs are performed at subsequent donations. Samples from the first 2217 participants were cultured using a two-step method to evaluate the effect of using enrichment broth. Furthermore, 262 participants were sampled from both the nares and the throat. All participants completed a questionnaire with self-reported health, anthropometric measurements, current smoking status, and physical activity. Plasma samples, nasal swab transport media, and isolates were stored.
The prevalence of nasal colonization was 41%. The prevalence of colonization was higher in men (46%) than women (34%), lower for smokers, and decreased with increasing age (<25 years: 44% vs >55 years: 35%). In participants swabbed from the nose and throat, the prevalence of colonization after enrichment was 55% with significantly higher prevalence in the throat (45%) than in the nose (40%). The use of an enrichment broth increased the proportion of colonization.
We describe a large and growing cohort of healthy individuals established to investigate predictors for carriage and the health consequences of carriage. Multiple projects using data from DBDSaCS linked with Danish health registers, biomarkers, and genetic markers are ongoing. Results will be published in the coming years.
携带[具体病原体名称未给出]会增加感染风险。本研究旨在调查健康个体中[具体病原体名称未给出]的定植情况，并建立一个前瞻性队列和生物样本库，以研究定植对健康的影响。
丹麦献血者[具体病原体名称未给出]定植研究（DBDSaCS）于2014年设立。到目前为止，共有6082名健康参与者纳入研究，采集鼻拭子样本，并在后续献血时重复采集拭子样本。对前2217名参与者的样本采用两步法进行培养，以评估使用增菌肉汤的效果。此外，从262名参与者的鼻腔和咽喉部位采集样本。所有参与者均完成一份关于自我报告的健康状况、人体测量、当前吸烟状况和身体活动情况的问卷。血浆样本、鼻拭子运输培养基和[具体病原体名称未给出]分离株均予以保存。
[具体病原体名称未给出]鼻定植率为41%。男性定植率（46%）高于女性（34%），吸烟者定植率较低，且随年龄增长而降低（<25岁：44% vs >55岁：35%）。在鼻腔和咽喉均采集样本的参与者中，增菌后[具体病原体名称未给出]定植率为55%，咽喉部定植率（45%）显著高于鼻腔（40%）。使用增菌肉汤可提高[具体病原体名称未给出]定植比例。
我们描述了一个规模庞大且不断扩大的健康个体队列，旨在研究[具体病原体名称未给出]携带的预测因素及其携带对健康的影响。目前正在进行多个利用DBDSaCS数据与丹麦健康登记册、生物标志物和基因标记相联系的项目。结果将在未来几年公布。
