Machado Laís Feitosa, de Assis Leite Deborah Catharine, da Costa Rachid Caio Tavora Coelho, Paes Jorge Eduardo, Martins Edir Ferreira, Peixoto Raquel Silva, Rosado Alexandre Soares
Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Research Center Leopoldo Américo Miguez de Mello, Rio de Janeiro, Brazil.
Front Microbiol. 2019 Sep 13;10:2107. doi: 10.3389/fmicb.2019.02107. eCollection 2019.
In this study, oil spills were simulated in field-based mangrove mesocosms to compare the efficiency of bioremediation strategies and to characterize the presence of the B, , A, and A genes and the ecological structures of microbial communities in mangrove sediments at two different depths, (D1) 1-10 cm and (D2) 25-35 cm. The results indicated that the hydrocarbon degradation efficiency was higher in superficial sediment layers, although no differences in the hydrocarbon degradation rates or in the abundances of the B and genes were detected among the tested bioremediation strategies at this depth. Samples from the deeper layer exhibited higher abundances of the analyzed genes, except for A and A, which were not detected in our samples. For all of the treatments and depths, the most abundant phyla were Proteobacteria, Firmicutes and Bacteroidetes, with Gammaproteobacteria, Flavobacteriales and Clostridiales being the most common classes. The indicator species analysis (ISA) results showed strong distinctions among microbial in response to different treatments and in the two collection depths. Our results indicated a high efficiency of the monitored natural attenuation (MNA) for oil consumption in the tested mangrove sediments, revealing the potential of this strategy for environmental decontamination and suggesting that environmental and ecological factors may select for specific bacterial populations in distinct niches.
在本研究中,在基于实地的红树林中宇宙模拟了石油泄漏,以比较生物修复策略的效率,并表征红树林沉积物中两个不同深度(D1)1 - 10厘米和(D2)25 - 35厘米处B、、A和A基因的存在以及微生物群落的生态结构。结果表明,表层沉积物层中的烃降解效率较高,尽管在此深度的测试生物修复策略之间未检测到烃降解率或B和基因丰度的差异。除了在我们的样本中未检测到的A和A外,来自较深层的样本显示出分析基因的丰度较高。对于所有处理和深度,最丰富的门是变形菌门、厚壁菌门和拟杆菌门,其中γ-变形菌纲、黄杆菌目和梭菌目是最常见的纲。指示物种分析(ISA)结果表明,微生物对不同处理和两个采集深度的反应存在强烈差异。我们的结果表明,在测试的红树林沉积物中,监测到的自然衰减(MNA)对石油消耗具有很高的效率,揭示了该策略在环境净化方面的潜力,并表明环境和生态因素可能会在不同生态位中选择特定的细菌种群。