Wiegand V, Henniges H, Oberschmidt R, Kreuzer H
Basic Res Cardiol. 1985 Jan-Feb;80(1):12-7. doi: 10.1007/BF01906739.
The influence of hyper- and hypothyroidism on atrial and ventricular myosin structure and Ca2+-activated ATPase activity has been analyzed in adult mini-pigs. Whereas no difference could be demonstrated between hypo- and euthyroid ventricular myocardium, Ca2+-activated ATPase activity was significantly higher in the hyperthyroid than in the hypo- or euthyroid state. Using pyrophosphate electrophoresis and isoelectric focusing of subfragment 1 this could be ascribed to an additional ventricular myosin in the hyperthyroid myocardium. Atrial myosin ATPase activity and structure were not influenced by the thyroid state of the animals. These results present evidence that thyreotoxicosis induces an additional isomyosin in the pig ventricular myocardium, albeit to a lesser degree than in the rodent heart. The lacking difference between the hypothyroid and the euthyroid states indicates that a myosin with a lower enzymatic activity than the normal ventricular myosin is not synthesized in the heart of higher mammals.
在成年小型猪中分析了甲状腺功能亢进和减退对心房和心室肌球蛋白结构以及钙离子激活的ATP酶活性的影响。甲状腺功能减退和甲状腺功能正常的心室心肌之间未显示出差异，而甲状腺功能亢进状态下钙离子激活的ATP酶活性显著高于甲状腺功能减退或甲状腺功能正常状态。通过焦磷酸电泳和亚片段1的等电聚焦分析发现，这可归因于甲状腺功能亢进心肌中存在额外的心室肌球蛋白。动物的甲状腺状态对心房肌球蛋白ATP酶活性和结构没有影响。这些结果表明，甲状腺毒症会在猪心室心肌中诱导产生额外的同功肌球蛋白，尽管程度低于啮齿动物心脏。甲状腺功能减退和甲状腺功能正常状态之间缺乏差异表明，高等哺乳动物心脏中不会合成酶活性低于正常心室肌球蛋白的肌球蛋白。
