染色质重塑因子 Fft3 在受阻的 DNA 复制叉处发挥双重作用。

Institut Curie, Paris-Saclay University, Centre National de la Recherche Scientifique, Unités Mixtes de Recherche 3348, F-91405, Orsay, France.

Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.

Life Sci Alliance. 2019 Oct 1;2(5). doi: 10.26508/lsa.201900433. Print 2019 Oct.

Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for single-strand annealing of an induced double-strand break. Furthermore, we use an inducible replication fork barrier system to show that Fft3 has two distinct roles at blocked DNA replication forks. First, Fft3 is needed for the resection of nascent strands, and second, it is required to restart the blocked forks. The latter function is independent of its ATPase activity.

在这里，我们研究了裂殖酵母 Fun30/Smarcad1 家族的 SNF2 ATP 依赖型染色质重塑酶在 DNA 损伤修复中的功能。裂殖酵母中有三种 Fun30 同源物，即 Fft1、Fft2 和 Fft3。我们发现只有 Fft3 在 DNA 修复中具有功能，并且它是诱导双链断裂的单链退火所必需的。此外，我们使用可诱导的复制叉障碍系统表明，Fft3 在受阻的 DNA 复制叉处具有两个不同的作用。首先，Fft3 对于新生链的切除是必需的，其次，它需要重新启动受阻的叉。后一个功能与其 ATP 酶活性无关。