Vinmec Biobank, Hi-tech Center, Vinmec Healthcare System, 458 Minh Khai, Ha Noi, Vietnam.
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Chem Commun (Camb). 2019 Oct 15;55(83):12428-12446. doi: 10.1039/c9cc04661e.
DNA is an attractive molecular building block to construct nanoscale structures for a variety of applications. In addition to their structure and function, modification the DNA nanostructures by other molecules opens almost unlimited possibilities for producing functional DNA-based architectures. Among the molecules to functionalize DNA nanostructures, proteins are one of the most attractive candidates due to their vast functional variations. DNA nanostructures loaded with various types of proteins hold promise for applications in the life and material sciences. When loading proteins of interest on DNA nanostructures, the nanostructures by themselves act as scaffolds to specifically control the location and number of protein molecules. The methods to arrange proteins of interest on DNA scaffolds at high yields while retaining their activity are still the most demanding task in constructing usable protein-modified DNA nanostructures. Here, we provide an overview of the existing methods applied for assembling proteins of interest on DNA scaffolds. The assembling methods were categorized into two main classes, noncovalent and covalent conjugation, with both showing pros and cons. The recent advance of DNA-binding adaptor mediated assembly of proteins on the DNA scaffolds is highlighted and discussed in connection with the future perspectives of protein assembled DNA nanoarchitectures.
DNA 是构建各种应用纳米结构的有吸引力的分子构建模块。除了它们的结构和功能外,通过其他分子对 DNA 纳米结构进行修饰,为生产基于 DNA 的功能性结构提供了几乎无限的可能性。在用于功能化 DNA 纳米结构的分子中,由于其功能的多样性,蛋白质是最具吸引力的候选者之一。负载各种类型蛋白质的 DNA 纳米结构有望在生命和材料科学领域得到应用。当将感兴趣的蛋白质加载到 DNA 纳米结构上时,纳米结构本身充当支架,以特异性控制蛋白质分子的位置和数量。在构建可用的蛋白质修饰 DNA 纳米结构时,以高产量保留其活性的方式将感兴趣的蛋白质排列在 DNA 支架上的方法仍然是最具挑战性的任务。在这里,我们提供了一种用于在 DNA 支架上组装感兴趣的蛋白质的现有方法概述。组装方法分为非共价和共价偶联两类,两者都有其优缺点。突出强调了 DNA 结合接头介导的蛋白质在 DNA 支架上组装的最新进展,并结合蛋白质组装 DNA 纳米结构的未来前景进行了讨论。