Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-011, Japan.
Molecules. 2023 Jan 16;28(2):911. doi: 10.3390/molecules28020911.
Cellular compartmentalization plays an essential role in organizing the complex and multiple biochemical reactions in the cell. An artificial compartment would provide powerful strategies to develop new biochemical tools for material production and diagnosis, but it is still a great challenge to synthesize the compartments that encapsulate materials of interest while controlling their accurate locations, numbers, and stoichiometry. In this study, we evaluated chemical characteristics of a liposome-encapsulated compartment, which has great potential to locate various materials of interest with precise control of their locations and numbers in the compartment. A nanoliposome was constructed inside a ring-shaped DNA origami skeleton according to the method of Yang et al., and further equipped with a double-stranded DNA platform to assemble molecules of interest in the nanoliposome. Upon formation of the nanoliposome, a pH-sensitive fluorophore on the bridged platform showed little or no response to the pH change of the outer buffer, ensuring that the molecules assembled on the platform are effectively shielded from the outer environment. The ring-shaped DNA skeleton equipped with a double-stranded DNA platform allows spatial assembly of several functional molecules inside the nanoliposome to isolate them from the outer environment.
细胞区室化在组织细胞内复杂多样的生化反应中起着至关重要的作用。人工区室为开发新材料生产和诊断的新生化工具提供了强有力的策略，但合成封装感兴趣材料的区室，同时控制其准确位置、数量和化学计量，仍然是一个巨大的挑战。在这项研究中，我们评估了脂质体包裹区室的化学特性，该区室具有将各种感兴趣的材料定位在区室内的精确位置和数量的巨大潜力。根据 Yang 等人的方法，在环形 DNA 折纸骨架内构建纳米脂质体，并进一步配备双链 DNA 平台，以在纳米脂质体内组装感兴趣的分子。形成纳米脂质体后，桥接平台上的 pH 敏感荧光团对外界缓冲液 pH 值的变化几乎没有响应，从而确保组装在平台上的分子有效地与外界环境隔离。配备双链 DNA 平台的环形 DNA 骨架允许在纳米脂质体内空间组装几个功能分子，将它们与外界环境隔离。
