Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Micro Fabrication of the Ministry of Education, Department of Instrument Science and Technology, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Nanoscale. 2019 Oct 10;11(39):18426-18435. doi: 10.1039/c9nr06774d.
This work explored the application of matrix metalloproteinase 2-targeted superparamagnetic nanoprobes for magnetic resonance imaging (MRI), near infrared (NIR) fluorescence imaging and photodynamic therapy of tumors. PEG, PAMAM (G5) and matrix metalloproteinase 2 (MMP2) were attached to the surface of carboxylated Fe3O4 nanoparticles (NPs) using a chemical coupling method and then finally loaded with the photosensitizer chlorin e6 (Ce6). In vitro and in vivo experiments demonstrated that the Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes exhibited excellent stability, precise tumor targeting and biocompatibility. Furthermore, the fluorescence properties of Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes were analogous to Ce6 and could be employed for fluorescence imaging. Meanwhile, the Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes have also been shown to be effective as contrast agents for T2-weighted MRI. The target molecule MMP2 enhanced the tumor targeting ability of Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes. Additionally, the Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes significantly inhibited tumor growth compared with PBS and free Ce6. This work will inspire greater enthusiasm for the construction of multifunctional magnetic nanoplatforms for biomedical applications.
这项工作探索了基质金属蛋白酶 2 靶向超顺磁纳米探针在磁共振成像（MRI）、近红外（NIR）荧光成像和肿瘤光动力治疗中的应用。通过化学偶联法将 PEG、PAMAM（G5）和基质金属蛋白酶 2（MMP2）连接到羧基化 Fe3O4 纳米颗粒（NPs）的表面，然后将其与光敏剂氯代叶绿素 e6（Ce6）一起负载。体外和体内实验表明，Fe3O4-PEG-G5-MMP2@Ce6 纳米探针具有优异的稳定性、精确的肿瘤靶向性和生物相容性。此外，Fe3O4-PEG-G5-MMP2@Ce6 纳米探针的荧光性质与 Ce6 类似，可以用于荧光成像。同时，Fe3O4-PEG-G5-MMP2@Ce6 纳米探针也可以作为 T2 加权 MRI 的对比剂。靶分子 MMP2 增强了 Fe3O4-PEG-G5-MMP2@Ce6 纳米探针的肿瘤靶向能力。此外，与 PBS 和游离 Ce6 相比，Fe3O4-PEG-G5-MMP2@Ce6 纳米探针显著抑制了肿瘤生长。这项工作将激发人们对构建用于生物医学应用的多功能磁性纳米平台的更大热情。
