Yin Ting, Huang Peng, Gao Guo, Shapter Joseph G, Shen Yulan, Sun Rongjin, Yue Caixia, Zhang Chunlei, Liu Yanlei, Zhou Sui, Cui Daxiang
Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P.R. China.
School of Chemical and Physical Sciences, Flinders University, Bedford Park, Adelaide 5042, Australia.
Sci Rep. 2016 Nov 8;6:36187. doi: 10.1038/srep36187.
The development of targeted nanoprobes is a promising approach to cancer diagnostics and therapy. In the present work, a novel multifunctional photo/magnet-diagnostic nanoprobe (MNPs-PEG-FA@Ce6) has been developed. This nanoprobe is built using folic acid (FA), bifunctional polyethylene glycol (PEG) and photosensitizer chlorin e6 (Ce6). The MNPs-PEG-FA@Ce6 nanoprobes are superparamagnetic, can be synthesized on a large scale by a one-pot hydrothermal process without further surface modification and are stable in an aqueous environment for eight months. Compared with free Ce6 nanoprobes in vitro studies, the MNPs-PEG-FA@Ce6 nanoprobes significantly enhance cellular uptake efficiency and promote the effectiveness of photodynamic therapy (PDT) with the assistance of 633 nm laser irradiation. The unique nanoprobes show superior penetration and a retention time of more than six days with less accumulation in the liver allowing highly effective tumor recognition and monitoring. Additionally, there was little damage to healthy organs or tissues. These exciting new nanoprobes could be potential building blocks to develop new clinical therapies and translational medicine.
靶向纳米探针的开发是一种很有前景的癌症诊断和治疗方法。在本研究中，一种新型的多功能光/磁诊断纳米探针（MNPs-PEG-FA@Ce6）被开发出来。这种纳米探针是利用叶酸（FA）、双功能聚乙二醇（PEG）和光敏剂二氢卟吩e6（Ce6）构建而成。MNPs-PEG-FA@Ce6纳米探针具有超顺磁性，可通过一锅水热法大规模合成，无需进一步表面修饰，且在水性环境中稳定八个月。与游离Ce6纳米探针的体外研究相比，MNPs-PEG-FA@Ce6纳米探针在633 nm激光照射的辅助下显著提高细胞摄取效率并促进光动力疗法（PDT）的有效性。这种独特的纳米探针显示出优异的穿透性和超过六天的保留时间，在肝脏中的积累较少，从而能够实现高效的肿瘤识别和监测。此外，对健康器官或组织几乎没有损伤。这些令人兴奋的新型纳米探针可能是开发新的临床治疗方法和转化医学的潜在基石。
