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提取物可抑制……中的生物膜形成。

extract inhibits biofilm formation in .

作者信息

Kamiya Mariko, Mori Takeshi, Nomura Mio, Inagaki Takayuki, Nonogaki Tunemasa, Nagatsu Akito, Yamagishi Yuka, Mikamo Hiroshige, Ikeda Yoshiaki

机构信息

College of Pharmacy, Kinjo Gakuin University, Nagoya, Japan.

Department of Infection Control and Prevention, Aichi Medical University Hospital, Nagakute, Japan.

出版信息

Nagoya J Med Sci. 2019 Aug;81(3):439-452. doi: 10.18999/nagjms.81.3.439.

Abstract

is capable of biofilm formation. In this study, we investigated the effects of aqueous extract on growth and biofilm formation. Aqueous extracts significantly inhibited both bacterial growth and biofilm formation. However, methanolic extracts inhibited neither. Aqueous extracts were deactivated by heating but were not deactivated by light exposure. The ingredients retained the inhibitory effect on the bacterial growth and biofilm formation after ultrafiltration of aqueous extract. Furthermore, polyphenol-rich extracts inhibited bacterial growth, thus, polyphenols are possible to be an active ingredient. We observed the biofilm by scanning electron microscopy, and quantitative and qualitative differences in the biofilm and cells morphology. Interestingly, the biofilm treated aqueous extracts remained premature. We postulated that premature biofilm formation was due to the inhibition of swarming motility. Indeed, aqueous extracts inhibited swarming motility. These results demonstrate that growth and biofilm formation are inhibited by aqueous extracts.

摘要

能够形成生物膜。在本研究中,我们研究了水提取物对生长和生物膜形成的影响。水提取物显著抑制细菌生长和生物膜形成。然而,甲醇提取物均未产生抑制作用。水提取物经加热后失活,但经光照未失活。水提取物超滤后,其成分对细菌生长和生物膜形成仍具有抑制作用。此外,富含多酚的提取物抑制细菌生长,因此,多酚可能是活性成分。我们通过扫描电子显微镜观察生物膜,并观察到生物膜和细胞形态在数量和质量上的差异。有趣的是,经水提取物处理的生物膜仍处于早期阶段。我们推测早期生物膜形成是由于群体运动受到抑制。事实上,水提取物抑制了群体运动。这些结果表明,水提取物可抑制生长和生物膜形成。

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