Zhao Nanxin, Cai Rongfeng, Zhang Yuting, Wang Xiaoli, Zhou Nandi
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Materials (Basel). 2022 Apr 12;15(8):2815. doi: 10.3390/ma15082815.
Multidrug-resistant bacteria are increasing, particularly those embedded in microbial biofilm. These bacteria account for most microbial infections in humans. Traditional antibiotic treatment has low efficiency in sterilization of biofilm-associated pathogens, and thus the development of new approaches is highly desired. In this study, amino-modified hollow mesoporous silica nanoparticles (AHMSN) were synthesized and used as the carrier to load natural photosensitizer curcumin (Cur). Then glutaraldehyde (GA) and polyethyleneimine (PEI) were used to seal the porous structure of AHMSN by the Schiff base reaction, forming positively charged AHMSN@GA@PEI@Cur. The Cur delivery system can smoothly diffuse into the negatively charged biofilm of (). Then Cur can be released to the biofilm after the pH-gated cleavage of the Schiff base bond in the slightly acidic environment of the biofilm. After the release of the photosensitizer, the biofilm was irradiated by the blue LED light at a wavelength of 450 nm and a power of 37.4 mV/cm for 5 min. Compared with the control group, the number of viable bacteria in the biofilm was reduced by 98.20%. Therefore, the constructed pH-gated photosensitizer delivery system can efficiently target biofilm-associated pathogens and be used for photodynamic sterilization, without the production of antibiotic resistance.
多重耐药菌正在增加，尤其是那些存在于微生物生物膜中的细菌。这些细菌导致了人类的大多数微生物感染。传统抗生素治疗对生物膜相关病原体的杀菌效率较低，因此迫切需要开发新的方法。在本研究中，合成了氨基修饰的中空介孔二氧化硅纳米颗粒（AHMSN），并将其用作载体来负载天然光敏剂姜黄素（Cur）。然后使用戊二醛（GA）和聚乙烯亚胺（PEI）通过席夫碱反应封闭AHMSN的多孔结构，形成带正电荷的AHMSN@GA@PEI@Cur。Cur递送系统可以顺利扩散到带负电荷的（此处原文缺失相关细菌名称）生物膜中。然后在生物膜微酸性环境中席夫碱键发生pH门控裂解后，Cur可以释放到生物膜中。光敏剂释放后，用波长为450 nm、功率为37.4 mV/cm的蓝色LED光照射生物膜5分钟。与对照组相比，生物膜中活菌数量减少了98.20%。因此，构建的pH门控光敏剂递送系统可以有效地靶向生物膜相关病原体并用于光动力杀菌，且不会产生抗生素耐药性。
