[暴露于物理、化学或生物性质因素后哺乳动物细胞中的DNA复制。III. 5-氟脱氧尿苷诱导未被γ射线抑制的细胞中的DNA合成]

[DNA replication in mammalian cells after exposure to factors of a physical, chemical or biological nature. III. 5-Fluorodeoxyuridine induces DNA synthesis in cells not suppressed by gamma irradiation].

作者信息

Synzynys B I, Brozmanová J, Sklobovskaia M V, Saenko A S

出版信息

Tsitologiia. 1985 Feb;27(2):236-40.

Abstract

The influence of preincubation of HeLa and Chinese hamster V79 cells with fluorodeoxyuridine (FUdR, 10(-6) M) on DNA replication and molecular weight of nascent DNA was studied after gamma-irradiation with a dose as much as 10 Gy. The 60Co-radiation inhibits DNA synthesis in both HeLa and V79 cells by 30-40 per cent. The incubation with FUdR before irradiation suppresses the inhibitory effect of irradiation on DNA synthesis. It is suggested that differences in gamma-radiation inhibition of DNA synthesis may result from the FUdR-induced changes in chromatin structure, rather than from synchronization of cell growth. This suggestion is based on the observation that the radioresistant mode of DNA synthesis occurred 18 hours following the short-term (6 hours) incubation with FUdR in cell cultures differing from each other in almost 2-fold their cell longevity.

摘要

研究了用10 Gy剂量的γ射线照射前，将氟脱氧尿苷（FUdR，10⁻⁶ M）预孵育HeLa细胞和中国仓鼠V79细胞对DNA复制及新生DNA分子量的影响。⁶⁰Co辐射使HeLa细胞和V79细胞中的DNA合成均抑制30% - 40%。照射前用FUdR孵育可抑制辐射对DNA合成的抑制作用。有人提出，γ辐射对DNA合成的抑制差异可能是由于FUdR诱导的染色质结构变化，而非细胞生长同步化所致。这一观点基于以下观察结果：在细胞寿命相差近2倍的细胞培养物中，用FUdR短期（6小时）孵育18小时后出现了抗辐射的DNA合成模式。