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[γ射线照射诱导HeLa细胞产生耐辐射DNA合成]

[Gamma-irradiation induces in HeLa cells radioresistant DNA synthesis].

作者信息

Synzynys B I, Aminev A G, Konstantinova S A, Saenko A S

出版信息

Radiobiologiia. 1987 Jul-Aug;27(4):459-62.

PMID:2957722
Abstract

Cells of a suspension HeLa culture at the logarithmic phase of growth were exposed to 60Co-gamma-rays (5 Gy), incubated in the nutritious medium, and in 4 h subjected to repeated irradiation: the dose-response function and the dynamics of DNA synthesis inhibition were determined. It was shown that DNA synthesis was inhibited to a lesser extent after preirradiation, in other words, DNA synthesis was radioresistant. A correlation between this synthesis and reproductive cell death is discussed.

摘要

处于对数生长期的悬浮培养海拉细胞,经60Co-γ射线(5 Gy)照射后,在营养培养基中孵育,并在4小时后进行重复照射:测定了剂量反应函数和DNA合成抑制的动力学。结果表明,预照射后DNA合成的抑制程度较小,换句话说,DNA合成具有抗辐射性。本文讨论了这种合成与生殖细胞死亡之间的相关性。

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Radiobiologiia. 1987 Jul-Aug;27(4):459-62.
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