Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Department of Laboratory Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Med Sci Monit. 2019 Oct 5;25:7471-7479. doi: 10.12659/MSM.915464.
BACKGROUND The purpose of the present research is to analyze the effect of polyphenols and flavonoids substrat (PFS) from plants Calendula officinalis, Salvia fruticosa, Achillea millefolium, and propolis as immunomodulatory in the production of interleukin (IL)-1ß and IL-10 in peripheral blood leukocytes medium (PBLM) in patients who were diagnosed with mucositis of peri-implant tissue compared to patients with healthy implant tissue. It was hypothesized that IL-1ß and IL-10 contribute to the inflammation processes noticed in the diseases of peri-implant tissues. MATERIAL AND METHODS Sixty non-smoking patients were included in this study: patients with healthy implants (HP group) and patients with peri-implant mucositis (MP group). Peri-mucositis was diagnosed by radiologic and clinical examination. The PBLM from MP were treated with PFS at various concentrations. The levels of IL-10 and IL-1ß excreted by the PBLM stimulated and unstimulated with viable Porphyromonas gingivalis test-tube were committed by the enzyme amplified immunoassay sensitivity method. RESULTS Unstimulated and stimulated PBLM and treatment with 5.0 mg/mL or 10.0 mg/mL of PFS in the MP group produced significantly higher levels IL-10 (P<0.001) that analogous mediums of the HP group. The levels of IL-1ß decreased more considerably in the stimulated PBLM of the MP group than in those of HP group (P<0.001) after the treatment with PFS at only 10.0 mg/mL concentration. CONCLUSIONS Theses results suggest that the solution of PFS might offer a new potential for the development of a new therapeutic path to prevent and treat peri-implant mucositis.
本研究的目的是分析植物金盏花、鼠尾草、蓍草和蜂胶中的多酚和黄酮基质（PFS）作为免疫调节剂对患有种植体周围组织黏膜炎的患者外周血白细胞培养液（PBLM）中白细胞介素（IL）-1β和 IL-10 的产生的影响，与健康种植体组织的患者相比。据推测，IL-1β和 IL-10 有助于解释种植体周围组织疾病中观察到的炎症过程。材料和方法：本研究纳入了 60 名不吸烟患者：健康种植体患者（HP 组）和种植体周围黏膜炎患者（MP 组）。通过放射学和临床检查诊断为种植体周围黏膜炎。将 MP 的 PBLM 用不同浓度的 PFS 处理。通过酶放大免疫分析敏感性法测定经活牙龈卟啉单胞菌试管刺激和未刺激的 PBLM 分泌的 IL-10 和 IL-1β水平。结果：未刺激和刺激的 PBLM 以及在 MP 组中用 5.0mg/mL 或 10.0mg/mL 的 PFS 处理后，IL-10 水平显著升高（P<0.001），与 HP 组的类似培养基相比。在用 10.0mg/mL 浓度的 PFS 处理后，MP 组刺激的 PBLM 中 IL-1β水平下降幅度明显大于 HP 组（P<0.001）。结论：这些结果表明，PFS 溶液可能为开发预防和治疗种植体周围黏膜炎的新治疗途径提供新的潜力。
