College of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , PR China.
J Agric Food Chem. 2019 Oct 30;67(43):12013-12025. doi: 10.1021/acs.jafc.9b04620. Epub 2019 Oct 18.
The synthesis pathways of quorum sensing (QS) signal molecules and the mechanism of action of quorum sensing inhibitors (QSIs) have gained considerable attention as research topics in the field of food preservation. Here, was detected as the specific spoilage organism in large yellow croaker during 4 °C storage, and it produced the QS signal molecules autoinducer-2 (AI-2) and diketopiperazines (DKPs). Then, a cyclodipeptide synthase (CDPS) homologous gene, , was screened, and knockout and rescue results revealed that this gene was involved in DKP synthesis but not in AI-2 synthesis, and it also played an important role in QS. Furthermore, fish fillets and mutant strains were treated with resveratrol, and the results suggested that resveratrol was an ideal QSI for inhibition of DKPs production via the gene and reduced QS in , thus delaying the process of fish spoilage during chilling storage.
群体感应（QS）信号分子的合成途径和群体感应抑制剂（QSIs）的作用机制作为食品保鲜领域的研究课题受到了相当大的关注。在这里，在 4°C 储存期间被检测为大黄鱼的特定腐败菌，并且它产生了群体感应信号分子自诱导物-2（AI-2）和二酮哌嗪（DKPs）。然后，筛选出一种环二肽合酶（CDPS）同源基因 ，敲除和拯救结果表明，该基因参与 DKP 合成但不参与 AI-2 合成，并且在 QS 中也发挥重要作用。此外，用白藜芦醇处理鱼片和突变株，结果表明白藜芦醇是一种理想的 QSI，可通过基因抑制 DKP 的产生，并降低 ，从而延缓冷藏储存过程中鱼肉变质的过程。
