College of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, People's Republic of China.
Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, People's Republic of China.
Environ Technol. 2021 Apr;42(11):1679-1692. doi: 10.1080/09593330.2019.1677782. Epub 2019 Oct 17.
In this paper, laccase was immobilized with the adsorption-crosslinking method in which biochar was used as the carrier and glutaraldehyde was used as the crosslinking agent. Firstly, the optimal immobilization conditions and optimal operating conditions were investigated, and then the stability of both free laccase and immobilized laccase was compared. Finally, the 2,4-dichlorophenol contaminated soil was remedied with both free laccase and immobilized laccase, and the improvement on the remediation of the contaminated soil by immobilized laccase was analysed through the ecological evaluation. The results showed that in the optimal immobilization condition, the biochar with a particle size of 30 mesh should be selected, and glutaraldehyde with a volume fraction of 4% and 20 mL of laccase solution should be added to complete the 6-hour adsorption operation and 4-hour crosslinking operation. The stability of immobilized laccase was better than that of free laccase, and the thermal deactivation kinetic equation for the free laccase was ln = -0.7657 + 0.4344 and the thermal deactivation kinetic equation for the immobilized laccase was ln = -0.1048 + 0.0608, respectively. The degradation ability of immobilized laccase for 2-4 dichlorophenol was better than that of free laccase. The degradation rate of 2,4-dichlorophenol was 44.4% in the free laccase group and 64.6% in the immobilized laccase group. The ecological evaluation showed that the biochar-immobilized laccase had a positive effect on the soil ecological environment in the remediation process of the soil and can improve the remediation of the contaminated soil to some extent.
本文采用生物炭作为载体、戊二醛作为交联剂，通过吸附交联法固定化漆酶。首先，考察了最佳固定化条件和最佳操作条件，然后比较了游离漆酶和固定化漆酶的稳定性。最后，采用游离漆酶和固定化漆酶修复 2,4-二氯苯酚污染土壤，并通过生态评价分析固定化漆酶对污染土壤修复的改善作用。结果表明，在最佳固定化条件下，应选择粒径为 30 目的生物炭，添加体积分数为 4%、20 mL 的漆酶溶液，完成 6 小时吸附操作和 4 小时交联操作。固定化漆酶的稳定性优于游离漆酶，游离漆酶的热失活动力学方程为 ln =-0.7657 + 0.4344，固定化漆酶的热失活动力学方程为 ln =-0.1048 + 0.0608。固定化漆酶对 2-4 二氯苯酚的降解能力优于游离漆酶。游离漆酶组 2,4-二氯苯酚的降解率为 44.4%，固定化漆酶组为 64.6%。生态评价表明，生物炭-固定化漆酶在土壤修复过程中对土壤生态环境有积极影响，可以在一定程度上改善污染土壤的修复。
