Roueinfar Mina, Abraham Kevin M, Hong Ka Lok
Department of Pharmaceutical Sciences, Nesbitt School of Pharmacy, Department of Biology, College of Science and Engineering, and Department of Physics, College of Science and Engineering, Wilkes University, 84 W. South Street, Wilkes-Barre, Pennsylvania 18766, United States.
ACS Omega. 2019 Sep 20;4(14):16201-16208. doi: 10.1021/acsomega.9b02414. eCollection 2019 Oct 1.
Atrazine is a common herbicide that is widely used to control weed growth in both agricultural and residential settings. It has been shown to act as an endocrine disruptor that affects aquatic organisms. Rapid and low-cost monitoring methods for atrazine is the first step to mitigate its widespread persistency. Aptamers are small synthetic oligonucleotides that can assume a 3D structure to act as the molecular recognition element for a specific target of interest. Two different atrazine binding aptamers (R12.23 Trunc. and R12.45 Trunc.) have been identified from the same library design but with fundamentally different in vitro selection methodologies. While the R12.23 Trunc. has been utilized in immobilized biosensing platforms, it is unclear if in-solution-based applications would be suitable for both atrazine binding aptamers. This study provides the first insight of comparative in-solution binding profiles of the two atrazine binding aptamers. Based on our results, this information will be useful for future biosensing platform development utilizing the two aptamers.
莠去津是一种常见的除草剂，广泛用于控制农业和住宅环境中的杂草生长。它已被证明是一种影响水生生物的内分泌干扰物。快速且低成本的莠去津监测方法是减轻其广泛持久性的第一步。适体是小的合成寡核苷酸，可呈现三维结构以充当特定目标的分子识别元件。从相同的文库设计中已鉴定出两种不同的莠去津结合适体（R12.23截短体和R12.45截短体），但体外筛选方法根本不同。虽然R12.23截短体已用于固定化生物传感平台，但基于溶液的应用是否适用于这两种莠去津结合适体尚不清楚。本研究首次深入了解了这两种莠去津结合适体的比较溶液内结合概况。基于我们的结果，这些信息将有助于未来利用这两种适体开发生物传感平台。
