Chemistry Department, McGill University, 801 Sherbrooke St. W., Montreal (QC), H3A 0B8, Canada.
ChemSusChem. 2020 Jan 9;13(1):106-110. doi: 10.1002/cssc.201902752. Epub 2019 Oct 30.
The activity of β-glucosidases-the enzymes responsible for the final step in the enzymatic conversion of cellulose to glucose-can be maintained and manipulated under mechanochemical conditions in the absence of bulk solvent, either through an unexpected stabilization effect of inert surfaces, or by altering the enzymatic equilibrium. The reported results illustrate unique aspects of mechanoenzymatic reactions that are not observable in conventional aqueous solutions. They also represent the first reported strategies to enhance activity and favor either direction of the reaction under mechanochemical conditions.
β-葡萄糖苷酶的活性——负责纤维素酶解转化为葡萄糖的最后一步的酶——可以在无本体溶剂的机械化学条件下得以保持和调控，这或是通过惰性表面出乎意料的稳定作用，或是通过改变酶平衡来实现。所报道的结果说明了在常规水溶液中观察不到的机械酶反应的独特方面。它们也代表了在机械化学条件下增强活性和有利于反应任一方向的首个被报道的策略。
