Chinkers M, McKanna J A, Cohen S
J Cell Biol. 1979 Oct;83(1):260-5. doi: 10.1083/jcb.83.1.260.
The morphological effects of epidermal growth factor (EGF) on human carcinoma cells A-431 have been examined by scanning electron microscopy. These flat polygonal cells normally exhibit only small membrane folds, but show extensive ruffling and extension of filopodia within 5 min of exposure to EGF at 37 degrees C. This ruffling activity is transient, subsiding within another 5--15 min, but several other changes in surface morphology follow. Within the first hour of exposure to the hormone, the cell surface becomes exceedingly smooth and the nuclei seem to protrude above the plane of the otherwise thin monolayer, giving the cells a "fried egg" appearance. Cells at the edges of colonies gradually retract from the substrate, leading to reorganization, by 12 h, of the monolayer into multilayered colonies. EGF thus induces both rapid and long-term alterations in the morphology of these epidermoid cells.
通过扫描电子显微镜检查了表皮生长因子（EGF）对人癌细胞A - 431的形态学影响。这些扁平的多边形细胞通常仅表现出小的膜褶皱，但在37℃下暴露于EGF 5分钟内就会出现广泛的皱褶和丝状伪足的延伸。这种皱褶活动是短暂的，在另外5 - 15分钟内消退，但随后表面形态会发生其他几种变化。在暴露于该激素的第一小时内，细胞表面变得极其光滑，细胞核似乎突出于原本很薄的单层平面之上，使细胞呈现出“煎蛋”外观。菌落边缘的细胞逐渐从基质上缩回，导致单层在12小时内重新组织成多层菌落。因此，EGF诱导这些表皮样细胞的形态发生快速和长期的改变。
