Malick L E, Wilson R B
Stain Technol. 1975 Jul;50(4):265-9. doi: 10.3109/10520297509117069.
A modified thiocarbohydrazide (TCH) technique to cross-link osmium layers has resulted in a reliable method for preparing a wide variety of soft biological tissues for scanning electron microscopy without the use of evaported metal. The technique works equally well on tissues with smooth surfaces and those with abrupt changes in contour or cut surfaces, as in biopsy or autopsied material or pathologically altered tissues, and thus has wide applicability. Small surface structures and junctional areas between cells are distinct. In addition, thin cells such as alveolar endothelium in the lung exhibit a transparent property, allowing the visualization of cells within the capillary lumen while retaining adequate contrast for study of the capillary wall itself. Absence of an evaporated metal coat makes the removal of tissue from the scanning electron microscope specimen easy for embedding and examination of the same material with the transmission electron microscope.
一种改良的硫代碳酰肼（TCH）技术用于交联锇层，已产生一种可靠的方法，可在不使用蒸发金属的情况下为扫描电子显微镜制备各种软生物组织。该技术在表面光滑的组织以及轮廓或切面有突然变化的组织（如活检或尸检材料或病理改变的组织）上同样有效，因此具有广泛的适用性。小的表面结构和细胞间的连接区域清晰可见。此外，诸如肺中的肺泡内皮等薄细胞具有透明特性，可在保留足够对比度以研究毛细血管壁本身的同时，使毛细血管腔内的细胞可视化。没有蒸发金属涂层使得从扫描电子显微镜标本中取出组织以便用透射电子显微镜对同一材料进行包埋和检查变得容易。
