Escande M L, Gas N, Stevens B J
Biol Cell. 1985;53(2):99-109. doi: 10.1111/j.1768-322x.1985.tb00359.x.
The localization of a major nucleolar protein with a molecular weight of 100,000 has been followed in Chinese hamster ovary cells using specific antibodies to this protein and immunocytochemical techniques. By immunofluorescence and immunocytochemistry at ultrastructural level in situ, the 100 K protein was detected abundantly in the nucleolus of interphase cells. In exponentially growing cells, the dense fibrillar component was shown to contain more 100 K protein than the granular RNP component but both the nucleolar components were positively immunostained. Fibrillar centers consistently showed weaker or no staining. The 100 K association with the preribosomal RNP components of the interphase nucleolus supports evidence for a role of this protein in pre-rRNA transcription and preribosomal processing.
利用针对分子量为100,000的主要核仁蛋白的特异性抗体和免疫细胞化学技术，在中国仓鼠卵巢细胞中追踪了该蛋白的定位。通过原位免疫荧光和超微结构水平的免疫细胞化学方法，在间期细胞核仁中大量检测到了100K蛋白。在指数生长期的细胞中，致密纤维成分所含的100K蛋白比颗粒状核糖核蛋白成分更多，但这两种核仁成分均呈阳性免疫染色。纤维中心始终显示较弱的染色或无染色。100K蛋白与间期核仁的前核糖体核糖核蛋白成分相关，这支持了该蛋白在rRNA前体转录和前核糖体加工中起作用的证据。
