Gas N, Inchauspé G, Azum M C, Stevens B
Exp Cell Res. 1984 Apr;151(2):447-57. doi: 10.1016/0014-4827(84)90394-x.
A major nucleolar protein in Chinese hamster ovary cells with a molecular weight (MW) of 100 kD has been found to stain selectively with the bismuth tartrate technique of Locke & Huie [19]. After glutaraldehyde fixation and bismuth staining of electrophoretic transfers of total nucleolar proteins separated by SDS-PAGE, a single band corresponding to the 100 kD protein is revealed. When the technique is applied to whole cells, small punctate regions of the nucleoli are strongly stained. At the ultrastructural level, bismuth selectively contrasts the fibrillar centers and the adjoining cords of the dense fibrillar component. The remainder of the dense fibrillar component is not stained. It is proposed that the high phosphorylation level of the 100 kD protein is responsible for its glutaraldehyde-insensitive bismuth staining. The concentration of this protein in certain localized regions of the nucleolus suggests that it plays a metabolic rather than a structural role.
在中国仓鼠卵巢细胞中发现一种分子量为100kD的主要核仁蛋白，用洛克和休伊[19]的酒石酸铋技术可对其进行选择性染色。用戊二醛固定经SDS-PAGE分离的总核仁蛋白的电泳转移产物并进行铋染色后，可显示出一条对应于100kD蛋白的单条带。当该技术应用于完整细胞时，核仁的小斑点区域会被强烈染色。在超微结构水平上，铋选择性地使纤维中心和致密纤维组分的相邻索带形成反差。致密纤维组分的其余部分未被染色。有人提出，100kD蛋白的高磷酸化水平是其对戊二醛不敏感的铋染色的原因。该蛋白在核仁某些局部区域的集中表明它起代谢作用而非结构作用。
