ICMN Interfaces, Confinement, Matériaux et Nanostructures, UMR7374 - Université d'Orléans-CNRS, 1b rue de la Férollerie, 45071, Orléans Cedex 2, France; Chemistry Department of Natural Sciences Faculty, University of Prishtina, rr. "Nëna Tereze" nr. 5, 10000, Prishtina, Kosovo.
ICMN Interfaces, Confinement, Matériaux et Nanostructures, UMR7374 - Université d'Orléans-CNRS, 1b rue de la Férollerie, 45071, Orléans Cedex 2, France.
Talanta. 2020 Jan 15;207:120222. doi: 10.1016/j.talanta.2019.120222. Epub 2019 Aug 5.
Isoproturon-imprinted polypyrrole films were electrochemically synthesized onto glassy carbon (GC) electrodes in an ethanol/aqueous solution of pyrrole as a monomer, isoproturon as a template molecule and LiClO as supporting electrolyte. Electropolymerization was performed by cyclic voltammetry and chronoamperometry. The isoproturon template molecules were successfully trapped in the polypyrrole film where they created artificial recognition cavities. After the electrochemical extraction of the template, the polypyrrole film acted as a molecularly imprinted polymer (MIP) for the selective recognition of isoproturon whereas the non-imprinted polymer (NIP) film, made in the same conditions except for the presence of isoproturon, did not exhibit any interaction. The MIP and NIP films were characterized by cyclic voltammetry in the presence of redox probes and the thickness of the polymer layers was estimated by EQCM (Electrochemical Quartz Crystal Microbalance) and calculated using Faraday's law. The isoproturon-imprinted polypyrrole films were found to selectively detect isoproturon even in the presence of the interferents carbendazim and carbamazepine. Its limit of detection (LOD) in milli Q water, achieved via square wave voltammetry was as low as 0.5 μg L, whereas in real water samples it was found to be 2.2 μg L.
在乙醇/水溶液中，以吡咯为单体、异丙隆为模板分子、LiClO4 为支持电解质，通过循环伏安法和恒电流安培法在玻碳电极上电化学合成了对异丙隆印迹的聚吡咯膜。模板分子异丙隆成功地被捕获在聚吡咯膜中，在那里形成了人工识别空腔。模板电化学提取后，聚吡咯膜作为分子印迹聚合物（MIP）对异丙隆具有选择性识别能力，而在相同条件下制备的非印迹聚合物（NIP）膜则没有表现出任何相互作用。通过在存在氧化还原探针的情况下进行循环伏安法对 MIP 和 NIP 膜进行了表征，并通过 EQCM（电化学石英晶体微天平）估计聚合物层的厚度，并根据法拉第定律进行计算。发现异丙隆印迹的聚吡咯膜即使在存在干扰物质苯并咪唑和卡马西平的情况下也能选择性地检测异丙隆。通过方波伏安法在毫 Q 水中的检测限（LOD）低至 0.5μg/L，而在实际水样中的检测限为 2.2μg/L。
