Department of Gastroenterology, School of Medicine, Toho University, Tokyo, Japan.
Department of Clinical Oncology, Graduate School of Medicine, Toho University, Tokyo, Japan.
Esophagus. 2020 Apr;17(2):168-174. doi: 10.1007/s10388-019-00698-5. Epub 2019 Oct 8.
Tumor budding is known predictors of lymph node metastasis from esophageal squamous cell carcinoma. However, it is not easy to detect such small cell clusters on hematoxylin-eosin (HE) staining. Therefore, we evaluated tumor budding using immunohistochemistry (IHC) for epithelial cell markers.
We analyzed tumor budding in 50 cases of superficial esophageal squamous cell carcinoma. We evaluated the impact of clinicopathological factors and tumor budding to predict lymph node metastasis. A total of 565 tumor sections were assessed using HE staining and IHC for cytokeratin 5/6.
Based on receiver operating characteristic curves, the cut-off values for high-grade tumor budding evaluated using HE staining or IHC were 2 and 11, respectively. High-grade tumor budding evaluated using HE staining (P = 0.007) and IHC (P ≤ 0.001) were significantly correlated with lymph node metastasis. For tumors with pT1a-MM to pT1b-SM1, high-grade tumor budding evaluated using IHC was correlated with lymph node metastasis (P = 0.050).
Tumor budding was significantly associated with lymph node metastasis. The optimal cut-off values of tumor budding on HE staining and tumor budding on IHC were 2 and 11, respectively. Even though both tumor budding on HE staining and tumor budding on IHC were significantly associated with lymph node metastasis, tumor budding on IHC tend to be more associated with lymph node metastasis.
肿瘤芽殖是食管鳞癌淋巴结转移的已知预测因子。然而，在苏木精-伊红（HE）染色上很难检测到这种小细胞簇。因此，我们使用上皮细胞标志物的免疫组织化学（IHC）来评估肿瘤芽殖。
我们分析了 50 例浅表性食管鳞癌中的肿瘤芽殖。我们评估了临床病理因素和肿瘤芽殖对预测淋巴结转移的影响。总共评估了 565 个肿瘤切片，使用 HE 染色和 IHC 评估细胞角蛋白 5/6。
基于接收者操作特征曲线，HE 染色或 IHC 评估的高级别肿瘤芽殖的截断值分别为 2 和 11。HE 染色（P=0.007）和 IHC（P≤0.001）评估的高级别肿瘤芽殖与淋巴结转移显著相关。对于 pT1a-MM 至 pT1b-SM1 的肿瘤，IHC 评估的高级别肿瘤芽殖与淋巴结转移相关（P=0.050）。
肿瘤芽殖与淋巴结转移显著相关。HE 染色和 IHC 上的肿瘤芽殖的最佳截断值分别为 2 和 11。尽管 HE 染色上的肿瘤芽殖和 IHC 上的肿瘤芽殖均与淋巴结转移显著相关，但 IHC 上的肿瘤芽殖与淋巴结转移的相关性更强。
