Laboratorio de Arbovirus, Centro de Investigaciones en Enfermedades Tropicales, Universidad Industrial de Santander, Bucaramanga, Colombia.
Centro de Investigaciones Epidemiológicas, Escuela de Medicina, Universidad Industrial de Santander, Bucaramanga, Colombia.
Trop Med Int Health. 2019 Dec;24(12):1400-1410. doi: 10.1111/tmi.13311. Epub 2019 Oct 31.
To investigate the link between fluctuations in the prevalence of dengue virus (DENV) serotypes and the number of dengue cases in the metropolitan area of Bucaramanga, Santander State, Colombia, in the 2007-2010 and 2014-2017 periods.
Viruses were isolated from febrile patient samples by direct application to C6/36-HT cells and typed using monoclonal antibodies. We performed autocorrelation and cross-correlation analyses to determine whether fluctuations in the prevalence of DENV serotypes and dengue cases were correlated. Full envelope (E) gene sequences were employed to examine the genetic diversity of serotypes circulating by using a phylogenetic approach.
All four dengue virus serotypes were detected. DENV-1 was the dominant serotype in both periods followed by DENV-3 or DENV-2 depending on the period; DENV-4 was the least prevalent virus in both periods. Cross-correlation analyses suggest a temporal relation between the fluctuations in the prevalence of DENV serotypes, which were almost simultaneous (lag = 0) or related to recent past fluctuations (lag > 1.0) in the number of dengue cases. Data suggest that a sustained predominance of DENV-1, an increase of the DENV-4 prevalence, and a switch from DENV-3 to DENV-2 could be linked to an outbreak. Circulating viruses were grouped into Genotype V, Asia/American III and II for DENV-1, -2, -3 and -4, respectively; intragenotypic diversity was detected.
The present work highlights the need of comprehensive studies on dynamics of DENV in Colombia to understand transmission of dengue and evaluate the effectiveness of a vaccination programme.
探究 2007-2010 年和 2014-2017 年期间，哥伦比亚桑坦德省布卡拉曼加大都市区登革热病毒（DENV）血清型流行率波动与登革热病例数之间的关系。
从发热患者样本中直接接种 C6/36-HT 细胞分离病毒，并使用单克隆抗体进行分型。我们进行自相关和互相关分析，以确定 DENV 血清型流行率波动与登革热病例之间是否存在相关性。采用系统发育方法，通过全包膜（E）基因序列研究循环血清型的遗传多样性。
检测到所有 4 种登革热病毒血清型。DENV-1 在两个时期均为优势血清型，其次是 DENV-3 或 DENV-2，具体取决于时期；DENV-4 在两个时期均为最不常见的病毒。互相关分析表明，DENV 血清型流行率的波动之间存在时间关系，几乎同时（滞后=0）或与登革热病例数的近期波动（滞后>1.0）有关。数据表明，DENV-1 的持续优势、DENV-4 流行率的增加以及 DENV-3 向 DENV-2 的转变可能与暴发有关。循环病毒分别归类为 DENV-1、-2、-3 和 -4 的基因型 V、亚洲/美洲 III 和 II；检测到种内多样性。
本研究强调了哥伦比亚全面研究 DENV 动态的必要性，以了解登革热的传播并评估疫苗接种计划的效果。
