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基于全基因组单核苷酸多态性(SNP)和简单序列重复(SSR)基因型构建茄子核心种质库。

Construction of a core collection of eggplant ( L.) based on genome-wide SNP and SSR genotypes.

作者信息

Miyatake Koji, Shinmura Yoshimi, Matsunaga Hiroshi, Fukuoka Hiroyuki, Saito Takeo

机构信息

Institute of Vegetable and Floriculture Science (NIVFS), National Agriculture and Food Research Organization (NARO), 360 Kusawa, Ano, Tsu, Mie 514-2392, Japan.

Takii & Company, Limited, 1360 Hari, Konan, Shiga 520-3231, Japan.

出版信息

Breed Sci. 2019 Sep;69(3):498-502. doi: 10.1270/jsbbs.18202. Epub 2019 Jul 10.

DOI:10.1270/jsbbs.18202
PMID:31598083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6776151/
Abstract

A core collection of eggplant ( L.) was developed based on a dataset of genome-wide 831 SNP and 50 SSR genotypes analyzed in 893 accessions of eggplant genetic resources collected in the NARO Genebank using the Core Hunter II program. The 893 accessions were collected worldwide, mainly Asia. Genetic variation and population structure among the 893 eggplant accessions were characterized. The genetic diversity of the Asian accessions, especially the South Asian and Southeast Asian accessions, forming the center of diversity in eggplant, was higher than that of the other regions. The resulting core collection, World Eggplant Core (WEC) collection consisted of 100 accessions basically collected from the high genetic diversity countries. Based on the results of the cluster and STRUCTURE analyses with SNP genotypes, the WEC collection was divided into four clusters (S1-S4). Each cluster corresponds to a geographical group as below, S1; the European, American and African countries, S2; the East Asian countries, S3; the Southeast Asian countries, S4; the South Asian and Southeast Asian countries. The genotype and phenotype data of the WEC collection are available from the VegMarks database (https://vegmarks.nivot.affrc.go.jp/resource/), and seed samples are available from the NARO Genebank (https://www.gene.affrc.go.jp/databases-core_collections.php).

摘要

基于使用Core Hunter II程序对日本农业研究机构基因库收集的893份茄子遗传资源进行全基因组831个单核苷酸多态性(SNP)和50个简单序列重复(SSR)基因型分析的数据,构建了茄子核心种质库。这893份种质资源是在全球范围内收集的,主要来自亚洲。对这893份茄子种质资源的遗传变异和群体结构进行了表征。亚洲种质资源,特别是构成茄子多样性中心的南亚和东南亚种质资源的遗传多样性高于其他地区。由此产生的核心种质库——世界茄子核心(WEC)种质库由100份种质组成,这些种质基本上是从遗传多样性高的国家收集而来。基于SNP基因型的聚类和结构分析结果,WEC种质库被分为四个聚类(S1 - S4)。每个聚类对应一个地理组,如下所示:S1为欧洲、美洲和非洲国家;S2为东亚国家;S3为东南亚国家;S4为南亚和东南亚国家。WEC种质库的基因型和表型数据可从VegMarks数据库(https://vegmarks.nivot.affrc.go.jp/resource/)获取,种子样本可从日本农业研究机构基因库(https://www.gene.affrc.go.jp/databases-core_collections.php)获取。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12ad/6776151/5e1994576908/69_18202_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12ad/6776151/5e1994576908/69_18202_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12ad/6776151/5e1994576908/69_18202_1.jpg

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