Vocational School of Health Services, Erzincan Binali Yıldırım University, Erzincan, Turkey.
Erzincan Horticultural Research Institute, Erzincan, Turkey.
Sci Rep. 2022 Apr 26;12(1):6814. doi: 10.1038/s41598-022-11005-1.
Plant genetic resources constitute the most valuable assets of countries. It is of great importance to determine the genetic variation among these resources and to use the data in breeding studies. To determine the genetic diversity among genotypes of Cucurbita pepo L. species of pumpkin, which is widely grown in Erzincan, 29 different pumpkin genotypes collected were examined based on the morphological parameters and molecular characteristics. SSR (Simple Sequence Repeat) markers were used to determine genetic diversity at the molecular level. The analysis of morphological characterization within genotypes showed a wide variability in morphological traits of plant, flower, fruit, and leaf. In the evaluation performed using SSR markers, all primers exhibited polymorphism rate of %100. Seven SSR markers yielded a total of 15 polymorphic bands, the number of alleles per marker ranged from 2 to 3, and the mean number of alleles was 2.14. Polymorphic information content (PIC) ranged from 0.06 (GMT-M61) to 0.247 (GMT-P41), and the mean PIC value per marker was 0.152. Cluster analysis using Nei's genetic distance determined that 29 genotypes were divided into 4 major groups. The present findings have revealed the genetic diversity among pumpkin genotypes collected from Erzincan province and may form the basis for further breeding studies in pumpkin.
植物遗传资源是各国最有价值的资产。确定这些资源之间的遗传变异并将数据用于育种研究非常重要。为了确定广泛种植于 Erzincan 的南瓜属 Cucurbita pepo L. 品种之间的遗传多样性，根据形态学参数和分子特征，对 29 个不同的南瓜基因型进行了研究。SSR（简单重复序列）标记用于确定分子水平的遗传多样性。对基因型内形态特征的分析表明，植物、花、果实和叶的形态特征存在广泛的变异性。在使用 SSR 标记进行的评估中，所有引物均表现出 100%的多态性率。7 个 SSR 标记共产生 15 个多态带，每个标记的等位基因数范围为 2-3，平均等位基因数为 2.14。多态信息含量（PIC）范围从 0.06（GMT-M61）到 0.247（GMT-P41），每个标记的平均 PIC 值为 0.152。基于 Nei 的遗传距离的聚类分析表明，29 个基因型分为 4 个主要组。本研究结果揭示了 Erzincan 省收集的南瓜基因型之间的遗传多样性，可为南瓜进一步的育种研究奠定基础。
