Yang Zhi, Gu Qingying, Wang Ying, Liu Bo, Zhou Guangming, Shao Chunlin, Ruan Jing, Jia Renbing, Ge Shengfang
Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai 200011, People's Republic of China.
J Cancer. 2019 Aug 28;10(21):5022-5030. doi: 10.7150/jca.30846. eCollection 2019.
Improving the effects of radiotherapy, such as heavy ion radiation, is currently a research priority for oncotherapy. Long non-coding RNAs (lncRNAs) are a subtype of noncoding RNAs involved in the therapeutic response to tumor radiotherapy. However, little is known about the variations in lncRNAs that occur after heavy ion radiation therapy. In this study, we established two kinds of Agilent Human lncRNA arrays and examined the effects of heavy ion radiation and X-ray irradiation on HeLa cells. We compared the differences in lncRNA expression (>=2-fold changes) between cells treated with the two types of radiation and control cells and identified 504 lncRNAs and 285 mRNAs that were differentially expressed. Among these lncRNAs, TCONS-00009910 was the most highly up-regulated lncRNA, while NONHSAT060631 was the most down-regulated lncRNA in both groups. To validate these sequencing data, RT-PCR was performed, and similar findings were obtained. GO and KEGG pathway analyses were employed to probe the potential functions of the affected lncRNAs. Numerous lncRNAs were changed after radiation exposure, showing that they may have important functions in the response to tumour radiotherapy. The present findings may help to elucidate the mechanism by which lncRNAs affect the clinical responses of cancer to radiation and may provide potential diagnostic and therapeutic targets for cancer therapy.
提高放疗效果，如重离子辐射，是目前肿瘤治疗研究的重点。长链非编码RNA（lncRNAs）是一类非编码RNA，参与肿瘤放疗的治疗反应。然而，对于重离子放疗后lncRNAs的变化知之甚少。在本研究中，我们建立了两种安捷伦人类lncRNA芯片，并检测了重离子辐射和X射线辐射对HeLa细胞的影响。我们比较了两种辐射处理的细胞与对照细胞之间lncRNA表达（变化≥2倍）的差异，鉴定出504个lncRNAs和285个差异表达的mRNA。在这些lncRNAs中，TCONS-00009910是上调最显著的lncRNA，而NONHSAT060631是两组中下调最显著的lncRNA。为了验证这些测序数据，进行了RT-PCR，并获得了相似的结果。采用GO和KEGG通路分析来探究受影响lncRNAs的潜在功能。辐射暴露后许多lncRNAs发生了变化，表明它们可能在肿瘤放疗反应中具有重要作用。本研究结果可能有助于阐明lncRNAs影响癌症放疗临床反应的机制，并可能为癌症治疗提供潜在的诊断和治疗靶点。
