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穿心莲主要农艺性状与二萜内酯含量的相关性分析

[Correlation analysis of main agronomic traits and contents of diterpene lactones of Andrographis paniculata].

作者信息

Zeng Wu-Jing, Xu Ling, He Qiu-Ling, Zhang Lan-Lan, Xu Hong-Liang, Liang Zong-Suo

机构信息

Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province,School of Life Sciences,Zhejiang Sci-Tech University Hangzhou 310018,China.

Digital Materia Medica Traditional Chinese Medicine Testing Co.,Ltd. Tianjin 300499,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2019 Aug;44(15):3233-3238. doi: 10.19540/j.cnki.cjcmm.20190527.109.

DOI:10.19540/j.cnki.cjcmm.20190527.109
PMID:31602877
Abstract

In order to study the correlation between the traits of Andrographis paniculata. The main agronomic traits and the content of four kinds of diterpene lactons were measured by the seedlings and the unmutagenized seeds carried by the spacecraft,and multiple comparisons,correlations and principal component analysis were carried out. The results showed that the agronomic traits of A. paniculata have different degrees of difference after being carried by space. The contents of diterpene lactones were quite different. The variation coefficients of deoxyandrographolide content,fresh weight,leaf dry weight,dehydrated andrographolide content,dry weight,neoandrographolide content and andrographolide content were all over 35%. There was a significant correlation between multiple traits,and the leaf weight ratio was significantly positively correlated with the number of primary tillers,leaf dry weight and dry weight,and was significantly negatively correlated with the content of deoxyandrographolide. Andrographolide content was a significantly negatively correlated with the number of leaves and the number of primary tillers,and positively correlated with the other three lactones. Five principal components were extracted from principal component analysis,and the cumulative contribution rate was 83. 127%,which were yield factor,plant type factor,leaf type factor,component factor and seed weight factor. Among the traits affecting the quality of A. paniculata,the yield factor for reliability of the selection of A. paniculata is higher than other factors. There are abundant variations among the traits of A. paniculata,carried in space which increase the genetic diversity. The principal component analysis method can be used to select the principal component factors according to the breeding requirements,which provides a theoretical basis for the breeding of high-yield and high-quality A. paniculata and the high-yield and stable cultivation of A. paniculata.

摘要

为研究穿心莲性状之间的相关性。对搭载航天器的穿心莲实生苗及未诱变种子的主要农艺性状和4种二萜内酯含量进行测定,并进行多重比较、相关性分析和主成分分析。结果表明,穿心莲经太空搭载后农艺性状存在不同程度差异,二萜内酯含量差异较大,其中去氧穿心莲内酯含量、鲜重、叶干重、脱水穿心莲内酯含量、干重、新穿心莲内酯含量和穿心莲内酯含量的变异系数均超过35%。多个性状间存在显著相关性,叶重比与一级分枝数、叶干重和干重呈显著正相关,与去氧穿心莲内酯含量呈显著负相关。穿心莲内酯含量与叶片数和一级分枝数呈显著负相关,与其他3种内酯呈正相关。主成分分析提取出5个主成分,累积贡献率为83.127%,分别为产量因子、株型因子、叶型因子、成分因子和粒重因子。在影响穿心莲品质的性状中,产量因子对穿心莲选育可靠性的影响高于其他因子。太空搭载的穿心莲性状间存在丰富变异,增加了遗传多样性。主成分分析方法可根据育种需求筛选主成分因子,为穿心莲高产优质育种及高产稳产栽培提供理论依据。

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