Zhao Qiu-Long, Bian Xiao-Kun, Qian Da-Wei, Zhang Ting, Zhu Zhen-Hua, Guo Sheng, Yan Hui, Wang Tuan-Jie, Chen Zhi-Peng, Duan Jin-Ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine Nanjing 210023,China.
National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine Nanjing 210023,China.
Zhongguo Zhong Yao Za Zhi. 2019 Aug;44(15):3316-3322. doi: 10.19540/j.cnki.cjcmm.20190424.202.
This study aims to compare the differences of Paeonia lactiflora from different habitats by establishing fingerprint. The fingerprint of P. lactiflora was established by UPLC. The samples collected from Sichuan,Hebei,Henan,Shanxi and Anhui were analyzed. The common peaks were identified by UPLC-Q-TOF/MS. The relative peak area of the common peaks was analyzed through similarity evaluation system( 2012 edition) for chromatographic fingerprint of traditional Chinese medicine developed by the National Pharmacopoeia Commission. Twelve common peaks were obtained and ten components were identified by reference substance and literature comparison. The similarity of each sample to the reference fingerprint is greater than 0. 900. However,all samples were clearly divided into 5 groups according to habitats after PLS-DA analysis. The peaks 2,6( ethyl gallate),10( galloypaeoniflorin) and 12( benzoyl paeoniflorin) were found to be the main difference components between the samples from five different habitats through the VIP value map. The study found that the variety of ingredients in the different areas are basically similar. But there are some differences in the content of the four components. The results of this study can provide reference at choosing and utilizing P. lactiflora from different places comprehensively.
本研究旨在通过建立指纹图谱比较不同产地芍药的差异。采用超高效液相色谱法（UPLC）建立芍药指纹图谱。对采自四川、河北、河南、山西和安徽的样品进行分析。通过超高效液相色谱-四极杆-飞行时间质谱联用仪（UPLC-Q-TOF/MS）对共有峰进行鉴定。运用国家药典委员会发布的《中药色谱指纹图谱相似度评价系统（2012版）》对共有峰的相对峰面积进行分析。共得到12个共有峰，通过对照品和文献比对鉴定出10个成分。各供试品指纹图谱与对照指纹图谱相似度均大于0.900。然而，经过偏最小二乘法判别分析（PLS-DA）后，所有样品根据产地明显分为5组。通过变量重要性投影（VIP）值图发现，峰2、6（没食子酸乙酯）、10（没食子酰芍药苷）和12（苯甲酰芍药苷）是来自5个不同产地样品之间的主要差异成分。研究发现，不同产地芍药的成分种类基本相似，但这4种成分的含量存在一定差异。本研究结果可为全面选择和利用不同产地的芍药提供参考。
