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Fluorescent complexes of DNA with DAPI 4',6-diamidine-2-phenyl indole.2HCl or DCI 4',6-dicarboxyamide-2-phenyl indole.DNA与4',6-二脒基-2-苯基吲哚二盐酸盐（DAPI）或4',6-二羧酰胺-2-苯基吲哚（DCI）的荧光复合物。
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2
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A new fluorometric method for RNA and DNA determination.一种用于RNA和DNA测定的新型荧光法。
Anal Biochem. 1966 Oct;17(1):100-7. doi: 10.1016/0003-2697(66)90012-1.
2
Interaction of nucleic acids. IV. The physical binding of 3,4-benzpyrene to nucleosides, nucleotides, nucleic acids, and nucleoprotein.核酸的相互作用。IV. 3,4-苯并芘与核苷、核苷酸、核酸及核蛋白的物理结合
Biochemistry. 1968 Jan;7(1):434-47. doi: 10.1021/bi00841a056.
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Corrections for instrumental errors in measurement of fluorescence and polarization of fluorescence.
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Biochemistry. 1971 Aug 3;10(16):3065-9. doi: 10.1021/bi00792a013.
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The binding of small cations to deoxyribonucleic acid. Nucleotide specificity.小阳离子与脱氧核糖核酸的结合。核苷酸特异性。
Biochemistry. 1969 Aug;8(8):3233-41. doi: 10.1021/bi00836a015.
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Interaction of the RNA polymerase inhibitor chromomycin with DNA.RNA聚合酶抑制剂放线菌素与DNA的相互作用。
Eur J Biochem. 1969 May 1;9(1):82-92. doi: 10.1111/j.1432-1033.1969.tb00579.x.
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Optical properties of the deoxyribonucleic acid-ethidium bromide complex. Effect of salt.脱氧核糖核酸-溴化乙锭复合物的光学性质。盐的影响。
Biochemistry. 1973 Mar 13;12(6):1213-21. doi: 10.1021/bi00730a031.
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Ethidium bromide as a cooperative effector of a DNA structure.溴化乙锭作为一种DNA结构的协同效应物。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3805-9. doi: 10.1073/pnas.69.12.3805.
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Evidence for sequence preferences in the intercalative binding of ethidium bromide to dinucleoside monophosphates.溴化乙锭与二核苷单磷酸嵌入结合中序列偏好性的证据。
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DNA与4',6-二脒基-2-苯基吲哚二盐酸盐（DAPI）或4',6-二羧酰胺-2-苯基吲哚（DCI）的荧光复合物。

Fluorescent complexes of DNA with DAPI 4',6-diamidine-2-phenyl indole.2HCl or DCI 4',6-dicarboxyamide-2-phenyl indole.

作者信息

Kapuściński J, Skoczylas B

出版信息

Nucleic Acids Res. 1978 Oct;5(10):3775-99. doi: 10.1093/nar/5.10.3775.

DOI:10.1093/nar/5.10.3775
PMID:31603
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342709/
Abstract

4',6-Dioarboxyamide-2-phenyl indole (DCI), a non-ionic structural analogue of 4',6-diamidine-2-phenyl indole.2HCl (DAPI), was synthesized in order to verify the hypothesis of intercalation of both dyes into the DNA double helix. The influence of pH, viscosity, and different concentrations of SDS (sodium dodecylsulphate) or NaCl on the optical and fluorescent properties and the changes in thermal transition of both dye complexes with DNA confirm the affinity of the dyes to the double helix as well as their stabilizing influence on the secondary DNA structure. The results of binding studies, carried out by fluorescent methods have shown that the dyes are strongly bound to DNA, though the number of binding sites is small. According to the experimental data, the fluorescent properties of DAPI and DCI complexes with DNA are connected with the intercalating binding mechanism of these dyes. On the other hand, the eventual ionic or hydrogen bonds of dyes outside the DNA helix do not change noticeably their fluorescent properties.

摘要

4',6-二羧酰胺基-2-苯基吲哚（DCI）是4',6-二脒基-2-苯基吲哚二盐酸盐（DAPI）的非离子结构类似物，其合成目的是验证这两种染料嵌入DNA双螺旋结构这一假说。pH值、粘度以及不同浓度的十二烷基硫酸钠（SDS）或氯化钠对两种染料与DNA形成的复合物的光学和荧光性质以及热转变变化的影响，证实了染料对双螺旋结构的亲和力以及它们对DNA二级结构的稳定作用。通过荧光方法进行的结合研究结果表明，尽管结合位点数量较少，但这些染料与DNA紧密结合。根据实验数据，DAPI和DCI与DNA形成的复合物的荧光性质与这些染料的嵌入结合机制有关。另一方面，染料在DNA螺旋外部最终形成的离子键或氢键对其荧光性质的影响并不明显。