Laboratoire d'Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria.
Laboratoire de Sécurité des Aliments, Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail (ANSES), Université PARIS-EST, Maisons-Alfort, France.
Microb Drug Resist. 2020 Apr;26(4):353-359. doi: 10.1089/mdr.2019.0249. Epub 2019 Oct 11.
This study aimed to characterize third-generation cephalosporin (3GC)-resistant isolated from fresh fruits and vegetables purchased at Bejaia city, Algeria. isolates were identified by MALDI-TOF. Susceptibility to antibiotics was tested by the disk diffusion method. Whole genome sequencing (WGS) was carried out to determine sequence type (ST), plasmid incompatibility group (Inc.), and acquired antimicrobial resistance gene presence. A total of 13 3GC-resistant strains were isolated. WGS identified in 11 extended-spectrum-beta-lactamases (ESBL)- and in 2 AmpC-. The gene was identified in 8 out of 13 isolates. Multilocus sequence typing (MLST) evidenced five different STs, namely ST14, ST45, ST219, ST236, and ST882. MDR contaminated fresh fruits and vegetables, often eaten raw and inappropriately washed, may represent an underestimated public health threat. This study highlights that hygiene measures during harvesting and retail process are of utmost importance to limit further ESBL/AmpC spread to the consumer households.
本研究旨在对从阿尔及利亚贝贾亚市购买的新鲜水果和蔬菜中分离出的第三代头孢菌素（3GC）耐药 进行鉴定。 使用 MALDI-TOF 进行 鉴定。 通过纸片扩散法测试抗生素的敏感性。 进行全基因组测序（WGS）以确定序列型（ST）、质粒不相容群（Inc.）和获得的抗微生物药物耐药基因的存在。 共分离出 13 株 3GC 耐药 株。 WGS 鉴定出 11 株为超广谱β-内酰胺酶（ESBL）和 2 株为 AmpC-。 在 13 株分离株中有 8 株检测到 基因。 多位点序列分型（MLST）证实了 5 种不同的 ST，分别为 ST14、ST45、ST219、ST236 和 ST882。 MDR 污染的新鲜水果和蔬菜，常被生食且清洗不当，可能构成被低估的公共卫生威胁。 本研究表明，在收获和零售过程中采取卫生措施对于限制 ESBL/AmpC 进一步向消费者家庭传播至关重要。
